Validation Data Gallery
|Positive WB detected in||HL-60 cells, A549 cells, Raji cells, U-937 cells|
|Positive IP detected in||HL-60 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10500-1-AP targets ASC/TMS1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, bovine, canine, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ASC/TMS1 fusion protein Ag0774|
|Full Name||PYD and CARD domain containing|
|Calculated molecular weight||25 kDa|
|Observed molecular weight||22-25 kDa|
|GenBank accession number||BC004470|
|Gene ID (NCBI)||29108|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.
|Product Specific Protocols|
|WB protocol for ASC/TMS1 antibody 10500-1-AP||Download protocol|
|IHC protocol for ASC/TMS1 antibody 10500-1-AP||Download protocol|
|IF protocol for ASC/TMS1 antibody 10500-1-AP||Download protocol|
|IP protocol for ASC/TMS1 antibody 10500-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Silencing of TMS1/ASC promotes resistance to anoikis in breast epithelial cells.
Role of hMOF-dependent histone H4 lysine 16 acetylation in the maintenance of TMS1/ASC gene activity.
Br J Pharmacol
Rhein regulates redox-mediated activation of NLRP3 inflammasomes in intestinal inflammation through macrophage-activated crosstalk.
J Hazard Mater
Mechanism investigation on Bisphenol S-induced oxidative stress and inflammation in murine RAW264.7 cells: The role of NLRP3 inflammasome, TLR4, Nrf2 and MAPK.
Am J Transplant
Enhanced autophagy alleviated corneal allograft rejection via inhibiting NLRP3 inflammasome activity.
Loss of AIM2 expression promotes hepatocarcinoma progression through activation of mTOR-S6K1 pathway.