CoraLite®594-conjugated TNF Alpha Monoclonal antibody

TNF Alpha Monoclonal Antibody for

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

11H8A5

Cat no : CL594-69002

Synonyms

Cachectin, DIF, NeutraKine® TNF Alpha, TNF, TNF a, TNF alpha, TNFA, TNF-alpha, TNFSF2, Tumor necrosis factor



Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-69002 targets TNF Alpha in applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TNF Alpha fusion protein HZ-1014
Full Name tumor necrosis factor (TNF superfamily, member 2)
Gene Symbol TNF alpha
Gene ID (NCBI) 7124
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.

This antibody can be used to neutralize the bioactivity of TNF alpha.