NeutraKine™ TNF alpha Antibody

NeutraKine™ TNF alpha Monoclonal Antibody for Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Neutralization, ELISA

Conjugate

Unconjugated

CloneNo.

11H8A5

Cat no : 69002-1-Ig

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor



Tested Applications

NeutralizationMeasured by its ability to neutralize TNF-alpha inhibition of L-929 growth. The neutralization dose ND50 is typically 10-50ng/mL.

Product Information

69002-1-Ig targets NeutraKine™ TNF alpha in Neutralization, ELISA applications and shows reactivity with Human samples.

<0.1 EU/μg
Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human Humankine TNF alpha protein HZ-1014
Full Name tumor necrosis factor (TNF superfamily, member 2)
Calculated molecular weight
Observed molecular weight
GenBank accession number
Gene symbol TNF
Gene ID (NCBI) 7124
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer Sterile PBS.
ReconsitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

This antibody is used to Block/Neutralize TNF alpha protein.

Protocols

Product Specific Protocols
Neutralization protocol of NeutraKine™ TNF alpha antibody 69002-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols