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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human TNF alpha protein

Activity

Typically ≤ 0.5 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1014

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor

Validation Data Gallery

  • The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

  • The activity was determined by the dose- dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay

    The activity was determined by the dose- dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay

Technical Specifications

GeneID 7124
Species human
Expression HEK293
Activity Typically ≤ 0.5 ng/mL EC50
Purity >95%
Molecular Mass 17 kDa, non-disulfide bonded homotrimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human, mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile
1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane-bound protein and free soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced mostly by activated macrophages, CD4+ lymphocytes, NK cells, neutrophils, eosinophils, and neurons. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation (PMID: 20194223).

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor

Publications

SpeciesTitle
human

Am J Obstet Gynecol

Progesterone inhibits in vitro fetal membrane weakening.

Authors - Deepak Kumar
View Article
human

Acta Biochim Biophys Sin (Shanghai)

miR-101-3p induces vascular endothelial cell dysfunction by targeting tet methylcytosine dioxygenase 2.

Authors - Qiaoli Chen
View Article
human

Bioorg Med Chem Lett

One unique steroidal sapogenin obtained through the microbial transformation of ruscogenin by Phytophthora cactorum ATCC 32134 and its potential inhibitory effect on tissue factor (TF) procoagulant activity.

Authors - Nai-DongChen
View Article
human

Oncol Rep

Protein-bound polysaccharide-K augments the anticancer effect of fluoropyrimidine derivatives possibly by lowering dihydropyrimidine dehydrogenase expression in gastrointestinal cancers.

Authors - EijiMekata
View Article
human

PLoS One

Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.

Authors - BaharehPezeshkian
View Article
human

Life Sci

Cynaropicrin is dual regulator for both degradation factors and synthesis factors in the cartilage metabolism.

Authors - TeruakiMasutani
View Article