- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human TNF alpha protein
Cat no : HZ-1014
Synonyms
Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor
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Technical Specifications
GeneID | 7124 |
Species | human |
Expression | HEK293 |
Activity | Typically ≤ 0.5 ng/mL EC50 |
Purity | >95% |
Molecular Mass | 17 kDa, non-disulfide bonded homotrimer, glycosylated |
Formulation | 1x PBS, See Certificate of Analysis for details |
Species Reactivity | human, mouse |
Stability and Reconstitution
Stability and Storage | Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products. |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
Background
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane-bound protein and free soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced mostly by activated macrophages, CD4+ lymphocytes, NK cells, neutrophils, eosinophils, and neurons. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation (PMID: 20194223).
Synonyms
Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor
Publications
Species | Title |
---|---|
Acta Biochim Biophys Sin (Shanghai) miR-101-3p induces vascular endothelial cell dysfunction by targeting tet methylcytosine dioxygenase 2. | |
Bioorg Med Chem Lett One unique steroidal sapogenin obtained through the microbial transformation of ruscogenin by Phytophthora cactorum ATCC 32134 and its potential inhibitory effect on tissue factor (TF) procoagulant activity. | |
Oncol Rep Protein-bound polysaccharide-K augments the anticancer effect of fluoropyrimidine derivatives possibly by lowering dihydropyrimidine dehydrogenase expression in gastrointestinal cancers. | |
PLoS One Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism. | |
Life Sci Cynaropicrin is dual regulator for both degradation factors and synthesis factors in the cartilage metabolism. |