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Animal-free Recombinant Human TNF alpha
Humankine®9 Publications

Catalog number: HZ-1014

HEK293 expressed Endotoxin-free Animal-component free

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Format: Lyophilized

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Technical Specifications

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TNF alpha activity assay

The activity was determined by the dose- dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay

TNF alpha purity

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. R represents reducing conditions and NR represents non-reducing conditions.

GeneID:
7124
Species:
Human
Expression:
HEK293
Activity:
Typically ≤1 ng/mL EC50
Purity:
>95%
Molecular Mass:
17 kDa, non-disulfide bonded homotrimer, glycosylated
Formulation
1x PBS, See Certificate of Analysis for details
Species Reactivity:
human, mouse

Stability and Reconstitution

Stability and Storage:
Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.


Reconstitution:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile
1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

Product Description

Product Description:

Animal-free Recombinant Human TNF alpha is expressed in human 293 cells and has been shown by gel filtration to be predominantly a non-covalently linked homotrimer, with a molecular mass of 51 kDa. The E. coli form is not a trimer. The cytokine is produced in a human cell express ion system with serum free, chemically defined media.



Background:

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane-bound protein and free soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced mostly by activated macrophages, CD4+ lymphocytes, NK cells, neutrophils, eosinophils, and neurons. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation (PMID: 20194223).



Synonyms:
Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin



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