• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human TNF alpha protein


0.002-0.026 ng/mL EC50

Species Reactivity




Cat no : HZ-1014


Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor

Technical Specifications

GeneID 7124
Species Human
Expression HEK293
Activity 0.002-0.026 ng/mL EC50
Purity >95%
Accession NumberP01375
Molecular Mass 17 kDa, non-disulfide bonded homotrimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile
1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).


TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane-bound protein and free soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced mostly by activated macrophages, CD4+ lymphocytes, NK cells, neutrophils, eosinophils, and neurons. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation (PMID: 20194223).


Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor



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