HumanKine® recombinant human TNF alpha protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

0.002-0.026 ng/mL EC50

Specific Activity

1.27x10^7 IU/mg calibrated against TNF alpha reference standard (NIBSC cat no: 17/232). Reference use only. Specific activity is not routinely tested

Species Reactivity

human,mouse

Purity

>95%

Publications

25

Reviews (1)

Cat no : HZ-1014

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor

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Validation Data Gallery

• 

  Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

• 

  Recombinant human TNF alpha (HZ-1014) demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL.

• 

  Proteintech TNF alpha demonstrates equivalent induction cytotoxicity and EC50 to leading competitors. TNF alpha (HZ-1014) demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL.

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Technical Specifications

• GeneID: 7124
• Species: Human
• Expression: HEK293
• Activity: 0.002-0.026 ng/mL EC50
• Specific Activity: 1.27x10^7 IU/mg calibrated against TNF alpha reference standard (NIBSC cat no: 17/232). Reference use only. Specific activity is not routinely tested
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P01375
• Molecular Mass: 17 kDa, non-disulfide bonded homotrimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

Time-Saving Dendritic Cell Generation No Medium Change - Less Reagents With HumanKine® GM-CSF and HumanKine IL4

Humankine® TNF Alpha: Authentic Trimer – High Efficiency, Human Cell Expressed

Background

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane-bound protein and free soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced mostly by activated macrophages, CD4+ lymphocytes, NK cells, neutrophils, eosinophils, and neurons. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation (PMID: 20194223).

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor

Publications

Species	Title
human	Nat Cell Biol; A20 promotes metastasis of aggressive basal-like breast cancers through multi-monoubiquitylation of Snail1.; Authors - Ji-Hyung Lee
human	Hepatology; Toll-like receptor 2-mediated innate immune response in human nonparenchymal liver cells toward adeno-associated viral vectors.; Authors - MariannaHösel
human	Biosens Bioelectron; Real-time monitoring of cell mechanical changes induced by endothelial cell activation and their subsequent binding with leukemic cell lines.; Authors - LiangTan
human	PLoS Pathog; Activation of HIV transcription by the viral Tat protein requires a demethylation step mediated by lysine-specific demethylase 1 (LSD1/KDM1).; Authors - NaokiSakane
human	Cell Prolif; Pin1 promotes pancreatic cancer progression and metastasis by activation of NF-κB-IL-18 feedback loop.; Authors - Qiqing Sun
human	Front Immunol; Therapeutic Effects of (5R)-5-Hydroxytriptolide on Fibroblast-Like Synoviocytes in Rheumatoid Arthritis via lncRNA WAKMAR2/miR-4478/E2F1/p53 Axis.; Authors - Xinpeng Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Susanne (Verified Customer) (08-12-2021)

The product was used to enhance the production of ROS.

• Applications: Cell culture
• Cell Tissue Type: HEK293