• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human TNF alpha protein


0.002-0.026 ng/mL

Specific Activity

minimally 1.00 x 107 IU/mg

Species Reactivity




Cat no : HZ-1014


Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor

Technical Specifications

GeneID 7124
Species Human
Expression HEK293
Activity(Primary) 0.002-0.026 ng/mL
Specific Activityminimally 1.00 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP01375
Molecular Mass 17-20 kDa reduced and non-reduced, non-disulfide bonded homotrimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse,rat

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.


TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane-bound protein and free soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced mostly by activated macrophages, CD4+ lymphocytes, NK cells, neutrophils, eosinophils, and neurons. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation (PMID: 20194223).


Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Susanne (Verified Customer) (08-12-2021)

The product was used to enhance the production of ROS.

  • Applications: Cell culture
  • Cell Tissue Type: HEK293