HumanKine® recombinant human TNF alpha protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

Typically ≤ 0.5 ng/mL EC50

Species Reactivity

human

Purity

>95%

Publications

20

Cat no : HZ-1014

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor

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Validation Data Gallery

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  The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

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  The activity was determined by the dose- dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay

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Technical Specifications

• GeneID: 7124
• Species: human
• Expression: HEK293
• Activity: Typically ≤ 0.5 ng/mL EC50
• Purity: >95%
• Accession Number: P01375
• Molecular Mass: 17 kDa, non-disulfide bonded homotrimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution	Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

Time-Saving Dendritic Cell Generation No Medium Change - Less Reagents With HumanKine® GM-CSF and HumanKine IL4

Humankine® TNF Alpha: Authentic Trimer – High Efficiency, Human Cell Expressed

Background

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane-bound protein and free soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced mostly by activated macrophages, CD4+ lymphocytes, NK cells, neutrophils, eosinophils, and neurons. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation (PMID: 20194223).

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor

Publications

Species	Title
human	Am J Obstet Gynecol; Progesterone inhibits in vitro fetal membrane weakening.; Authors - Deepak Kumar
human	Acta Biochim Biophys Sin (Shanghai); miR-101-3p induces vascular endothelial cell dysfunction by targeting tet methylcytosine dioxygenase 2.; Authors - Qiaoli Chen
human	J Exp Clin Cancer Res; Phosphorylation of NF-κBp65 drives inflammation-mediated hepatocellular carcinogenesis and is a novel therapeutic target.; Authors - Xuan Xu
human	Bioorg Med Chem Lett; One unique steroidal sapogenin obtained through the microbial transformation of ruscogenin by Phytophthora cactorum ATCC 32134 and its potential inhibitory effect on tissue factor (TF) procoagulant activity.; Authors - Nai-DongChen
human	Oncol Rep; Protein-bound polysaccharide-K augments the anticancer effect of fluoropyrimidine derivatives possibly by lowering dihydropyrimidine dehydrogenase expression in gastrointestinal cancers.; Authors - EijiMekata
human	PLoS One; Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.; Authors - BaharehPezeshkian

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