HVEM/TNFRSF14 Polyclonal antibody

HVEM/TNFRSF14 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10138-1-AP

Synonyms

ATAR, Herpes virus entry mediator A, Herpesvirus entry mediator A, HVEA, HVEM, LIGHTR, TNFRSF14, TR2



Tested Applications

Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10138-1-AP targets HVEM/TNFRSF14 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HVEM/TNFRSF14 fusion protein Ag0186
Full Name tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
Calculated molecular weight 30 kDa
GenBank accession numberBC002794
Gene symbol TNFRSF14
Gene ID (NCBI) 8764
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mammalian tumor necrosis factor receptor (TNFR) family consists of 10 cell-surface proteins that regulate development and homeostasis of the immune system. Member 14 of TNFR (TNFRSF14, also named as TR2, ATAR, HVEA, HVEM, LIGHTR) was also identified as a mediator of herpesvirus entry into mammalian cells. The cytoplasmic region of TNFRSF14 bound to several members of the TNFR-associated factor (TRAF) family, namely TRAF1, TRAF2, TRAF3, and TRAF5. Transient transfection into human 293 cells caused marked activation of nuclear factor- B (NF- B), a transcriptional regulator of multiple immunomodulatory and inflammatory genes.

Protocols

Product Specific Protocols
IHC protocol for HVEM/TNFRSF14 antibody 10138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

EBioMedicine

Immune checkpoint molecule herpes virus entry mediator is overexpressed and associated with poor prognosis in human glioblastoma.

Authors - Ming-Zhi Han
humanWB

Obesity (Silver Spring)

Characterization of herpes virus entry mediator as a factor linked to obesity.

Authors - Judit Bassols