TNFRSF17 Polyclonal antibody

TNFRSF17 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27724-1-AP

Synonyms

B cell maturation protein, BCM, BCMA, CD269, TNFRSF17

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Validation Data Gallery

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  WB analysis of Daudi using 27724-1-AP

  Daudi and Ramos cells were subjected to SDS PAGE followed by western blot with 27724-1-AP (TNFRSF17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 27724-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27724-1-AP (TNFRSF17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 27724-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 27724-1-AP (TNFRSF17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27724-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27724-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27724-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27724-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Daudi using 27724-1-AP

  1X10^6 Daudi cells were stained with 0.20 ug/test Anti-Human TNFRSF17 (27724-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells
• Positive IHC detected in: human breast cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: Daudi cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27724-1-AP targets TNFRSF17 in WB, IHC, FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNFRSF17 fusion protein Ag27049
• Full Name: tumor necrosis factor receptor superfamily, member 17
• Calculated Molecular Weight: 184 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC058291
• Gene Symbol: TNFRSF17
• Gene ID (NCBI): 608
• RRID: AB_2880954
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNFRSF17 (also known as BCMA and CD269) is located on chromosome 16p13.1 and was initially cloned as a translocation product in human T cell lymphom. TNFRSF17 is preferentially expressed in mature B lymphocytes. TNFRSF17 is involved in the late stages of B cell maturation; it is important for the survival of plasmablasts and long-lived plasma cells in the bone marrow.

Publications

Species	Application	Title
human	WB	J Exp Med; Developing high-affinity decoy receptors to treat multiple myeloma and diffuse large B cell lymphoma.; Authors - Yu Rebecca Miao