BCMA/TNFRSF17 Polyclonal antibody

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  WB analysis of Daudi using 27724-1-AP

  Daudi and Ramos cells were subjected to SDS PAGE followed by western blot with 27724-1-AP (TNFRSF17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 27724-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27724-1-AP (TNFRSF17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 27724-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 27724-1-AP (TNFRSF17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27724-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27724-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27724-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27724-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BCMA/TNFRSF17 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27724-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

TNFRSF17, CD269, BCMA, BCM, B-cell maturation protein

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells
• Positive IHC detected in: human breast cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

27724-1-AP targets BCMA/TNFRSF17 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27049

  Product name: Recombinant human TNFRSF17 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 78-184 aa of BC058291

  Sequence: RKINSEPLKDEFKNTGSGLLGMANIDLEKSRTGDEIILPRGLEYTVEECTCEDCIKSKPKVDSDHCFPLPAMEEGATILVTTKTNDYCKSLPAALSATEIEKSISAR

• Full Name: tumor necrosis factor receptor superfamily, member 17
• Calculated Molecular Weight: 184 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC058291
• Gene Symbol: TNFRSF17
• Gene ID (NCBI): 608
• RRID: AB_2880954
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02223
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCMA (B cell maturation antigen), also known as TNFRSF17, is 20.2-kDa type III transmembrane glycoprotein and is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes and plasma cells, which may be important for B cell development and autoimmune response (PMID: 32943087; 9846698). BCMA has two agonist ligands: a proliferation-inducing ligand (APRIL) and BAFF. When BCMA binds to APRIL, it transmits signals of cell survival and proliferation (PMID: 27127303); when BCMA binds to BAFF, it mediates the activation of NF-kappaB and MAPK8/JNK (PMID: 36140254). It has been found that the overexpression and activation of BCMA are associated with multiple myeloma (MM) in preclinical models and humans, supporting its potential utility as a therapeutic target for MM (PMID: 32055000; 32943087).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Eur J Nucl Med Mol Imaging; Discovery of a highly specific radiolabeled antibody targeting B-cell maturation antigen: Applications in PET imaging of multiple myeloma; Authors - Jie Ma
human,mouse	WB,IHC	BMC Cancer; 25-hydroxycholesterol promotes proliferation and metastasis of lung adenocarcinoma cells by regulating ERβ/TNFRSF17 axis; Authors - Mengting He ; KD Validated
human	WB	J Exp Med; Developing high-affinity decoy receptors to treat multiple myeloma and diffuse large B cell lymphoma.; Authors - Yu Rebecca Miao
human	WB	J Med Chem; Development of a Novel Peptide-Based PET Tracer [68Ga]Ga-DOTA-BP1 for BCMA Detection in Multiple Myeloma; Authors - Lele Song