Anti-Human TNFRSF21/DR6 Rabbit Recombinant Antibody

• 

  FC experiment of A431 using 98523-2-RR

  1x10^6 A431 cells were surface stained with 0.25 ug Anti-Human TNFRSF21/DR6 Rabbit RecAb (98523-2-RR, Clone: 250249D8) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of A431 using 98523-2-RR

  1x10^6 A431 cells were surface stained with 0.25 ug Anti-Human TNFRSF21/DR6 Rabbit RecAb (98523-2-RR, Clone: 250249D8) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of Transfected CHO using 98523-2-RR

  1x10^6 HEK-293T cells or TNFRSF21/DR6-transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human TNFRSF21/DR6 Rabbit RecAb (98523-2-RR, Clone: 250249D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of Transfected CHO using 98523-2-RR

  1x10^6 HEK-293T cells or TNFRSF21/DR6-transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human TNFRSF21/DR6 Rabbit RecAb (98523-2-RR, Clone: 250249D8) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

TNFRSF21/DR6 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98523-2-RR

Clone No.250249D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

DR6, TNFRSF21, CD358, Death receptor 6, UNQ437/PRO868

---

Tested Applications

• Positive FC detected in: A431 cells, Transfected CHO cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98523-2-RR targets TNFRSF21/DR6 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2718

  Product name: Recombinant Human TNFRSF21/DR6 protein (rFc Tag)(HPLC verified)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 42-349 aa of NM_014452.5

  Sequence: QPEQKASNLIGTYRHVDRATGQVLTCDKCPAGTYVSEHCTNTSLRVCSSCPVGTFTRHENGIEKCHDCSQPCPWPMIEKLPCAALTDRECTCPPGMFQSNATCAPHTVCPVGWGVRKKGTETEDVRCKQCARGTFSDVPSSVMKCKAYTDCLSQNLVVIKPGTKETDNVCGTLPSFSSSTSPSPGTAIFPRPEHMETHEVPSSTYVPKGMNSTESNSSASVRPKVLSSIQEGTVPDNTSSARGKEDVNKTLPNLQVVNHQQGPHHRHILKLLPSMEATGGEKSSTPIKGPKRGHPRQNLHKHFDINEH

• Full Name: tumor necrosis factor receptor superfamily, member 21
• Calculated Molecular Weight: 72 kDa
• GenBank Accession Number: NM_014452.5
• Gene Symbol: DR6
• Gene ID (NCBI): 27242
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75509
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

TNFRSF21 (also known as DR6 or CD358) is an orphan receptor of the tumor necrosis factor (TNF) receptor superfamily. It controls cell death and differentiation in a cell-autonomous manner in different cell types including neurons, oligodendrocytes, endothelial cells, and immune cells (PMID: 29459438). DR6 has been shown to activate NF-κB and JNK, and induce cell apoptosis (PMID: 9714541). DR6 interacts with TRADD, which is known to mediate signal transduction of TNF receptors. It has been reported that APP binds DR6 to trigger axon pruning and neuron death via distinct caspases, indicating that DR6 might be involved in Alzheimer's disease (PMID: 19225519).