Tested Applications
| Positive WB detected in | pig brain tissue, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells, human peripheral blood platelets, rabbit brain tissue, rat brain tissue, mouse brain tissue | 
| Positive IHC detected in | mouse colon tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 | 
| Positive IF/ICC detected in | U2OS cells | 
| Positive FC (Intra) detected in | Jurkat cells | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 | 
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 | 
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 | 
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68170-1-Ig targets TOLLIP in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig | 
| Host / Isotype | Mouse / IgG1 | 
| Class | Monoclonal | 
| Type | Antibody | 
| Immunogen | CatNo: Ag32731 Product name: Recombinant human TOLLIP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-274 aa of BC012057 Sequence: MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEPPredict reactive species | 
| Full Name | toll interacting protein | 
| Calculated Molecular Weight | 30 kDa | 
| Observed Molecular Weight | 30 kDa | 
| GenBank Accession Number | BC012057 | 
| Gene Symbol | TOLLIP | 
| Gene ID (NCBI) | 54472 | 
| RRID | AB_2923686 | 
| Conjugate | Unconjugated | 
| Form | Liquid | 
| Purification Method | Protein G purification | 
| UNIPROT ID | Q9H0E2 | 
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. | 
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. | 
Background Information
TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for TOLLIP antibody 68170-1-Ig | Download protocol | 
| IF protocol for TOLLIP antibody 68170-1-Ig | Download protocol | 
| IHC protocol for TOLLIP antibody 68170-1-Ig | Download protocol | 
| WB protocol for TOLLIP antibody 68170-1-Ig | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
















