TOLLIP Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP01524-2

  Sandwich ELISA standard curve of MP01524-2, Human TOLLIP Recombinant Matched Antibody Pair - PBS only. 84711-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1864. 84711-1-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

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  Sample test of MP01524-2

  The mean TOLLIP concentration was determined to be 32.07 ng/mL in HuH-7 cell extract based on a 1.4 mg/mL extract load.

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  Cytometric bead array standard curve of MP01524-2

  Cytometric bead array standard curve of MP01524-2, TOLLIP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84711-2-PBS. Detection antibody: 84711-1-PBS. Standard: Ag1864. Range: 0.469-60 ng/mL.

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  Affinity and Kinetic Characterization of 84711-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84711-1-PBS against Human TOLLIP was performed. The affinity constant is 1.19 nM.

TOLLIP Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84711-1-PBS

Clone No.242098A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

IL 1RAcPIP, toll interacting protein, Toll-interacting protein

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Product Information

84711-1-PBS targets TOLLIP as part of a matched antibody pair:

MP01524-2: 84711-2-PBS capture and 84711-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1864

  Product name: Recombinant human TOLLIP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-274 aa of BC012057

  Sequence: MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP

• Full Name: toll interacting protein
• Calculated Molecular Weight: 30 kDa
• GenBank Accession Number: BC012057
• Gene Symbol: TOLLIP
• Gene ID (NCBI): 54472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H0E2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.