|Positive WB detected in||HEK-293 cells, human brain tissue, A431 cells, HeLa cells, HepG2 cells|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human liver tissue, human heart tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18409-1-AP targets TOMM40 in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TOMM40 fusion protein Ag13065|
|Full Name||translocase of outer mitochondrial membrane 40 homolog (yeast)|
|Calculated molecular weight||38 kDa|
|Observed molecular weight||38 kDa|
|GenBank accession number||BC017224|
|Gene ID (NCBI)||10452|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
|Product Specific Protocols|
|WB protocol for TOMM40 antibody 18409-1-AP||Download protocol|
|IHC protocol for TOMM40 antibody 18409-1-AP||Download protocol|
|IF protocol for TOMM40 antibody 18409-1-AP||Download protocol|
|IP protocol for TOMM40 antibody 18409-1-AP||Download protocol|
|FC protocol for TOMM40 antibody 18409-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Reciprocal Roles of Tom7 and OMA1 during Mitochondrial Import and Activation of PINK1.
Amyloid beta-mediated KIF5A deficiency disrupts anterograde axonal mitochondrial movement.
The class 3 PI3K coordinates autophagy and mitochondrial lipid catabolism by controlling nuclear receptor PPARα.
TOM40 Inhibits Ovarian Cancer Cell Growth by Modulating Mitochondrial Function Including Intracellular ATP and ROS Levels.
Dependence of PINK1 accumulation on mitochondrial redox system.
Cell Death Dis
Oxidative stress-induced mitophagy is suppressed by the miR-106b-93-25 cluster in a protective manner.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (03-23-2021)
Cos7 cells fixed in 4% PFA and permeabilized in 0.1% Triton X-100. Cells blocked in 1% BSA in PBS.
Daniela (Verified Customer) (01-08-2020)
Immunoblot of total cell lysate of HeLa cell gives three bands, one specific for human Tom40 above 37 KDa on 15% acrylamide gel, the other two no specific and with much lower intensity. The anti-Tom40 antibody worked very well and specifically when conjugated with magnetic beads for immunoprecipitation.