• Featured Product
  • KD/KO Validated

TOMM40 Polyclonal antibody

TOMM40 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 18409-1-AP

Synonyms

C19orf1, D19S1177E, p38.5, PER EC1, PEREC1, Protein Haymaker, TOM40, TOMM40



Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, A431 cells, HeLa cells, HepG2 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18409-1-AP targets TOMM40 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOMM40 fusion protein Ag13065
Full Name translocase of outer mitochondrial membrane 40 homolog (yeast)
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC017224
Gene symbol TOMM40
Gene ID (NCBI) 10452
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TOMM40 antibody 18409-1-APDownload protocol
IHC protocol for TOMM40 antibody 18409-1-APDownload protocol
IF protocol for TOMM40 antibody 18409-1-APDownload protocol
IP protocol for TOMM40 antibody 18409-1-APDownload protocol
FC protocol for TOMM40 antibody 18409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Reciprocal Roles of Tom7 and OMA1 during Mitochondrial Import and Activation of PINK1.

Authors - Shiori Sekine
mouseWB

Neurobiol Dis

Amyloid beta-mediated KIF5A deficiency disrupts anterograde axonal mitochondrial movement.

Authors - Qi Wang
mouseIF

Nat Commun

The class 3 PI3K coordinates autophagy and mitochondrial lipid catabolism by controlling nuclear receptor PPARα.

Authors - Anton Iershov
humanWB

Cancers (Basel)

TOM40 Inhibits Ovarian Cancer Cell Growth by Modulating Mitochondrial Function Including Intracellular ATP and ROS Levels.

Authors - Wookyeom Yang
  • KD Validated
humanWB

Aging Cell

Dependence of PINK1 accumulation on mitochondrial redox system.

Authors - Feng Gao
humanWB

Toxicon

Changes in the mitochondrial proteome in human hepatocytes in response to alpha-amanitin hepatotoxicity.

Authors - Mei Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Daniela (Verified Customer) (01-08-2020)

Immunoblot of total cell lysate of HeLa cell gives three bands, one specific for human Tom40 above 37 KDa on 15% acrylamide gel, the other two no specific and with much lower intensity. The anti-Tom40 antibody worked very well and specifically when conjugated with magnetic beads for immunoprecipitation.

  • Applications: Western Blot,Immunoprecipitation,Cell culture,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells