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TOMM40 Polyclonal antibody
TOMM40 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 18409-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, human brain tissue, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human liver tissue, human kidney tissue, human heart tissue, mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HeLa cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
18409-1-AP targets TOMM40 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, monkey, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TOMM40 fusion protein Ag13065|
|Full Name||translocase of outer mitochondrial membrane 40 homolog (yeast)|
|Calculated molecular weight||38 kDa|
|Observed molecular weight||38 kDa|
|GenBank accession number||BC017224|
|Gene ID (NCBI)||10452|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The translocase of outer mitochondria membrane 40 (TOMM40, also known as TOM40), located in the center of the TOM complex, is a channel-forming subunit of translocase. It can facilitate the fluid movement of preproteins into the mitochondria by associating with TOMM20. TOMM40 plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PMID: 31206022). TOMM40 has been reported to be associated with late-onset neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.
|Product Specific Protocols|
|WB protocol for TOMM40 antibody 18409-1-AP||Download protocol|
|IHC protocol for TOMM40 antibody 18409-1-AP||Download protocol|
|IF protocol for TOMM40 antibody 18409-1-AP||Download protocol|
|IP protocol for TOMM40 antibody 18409-1-AP||Download protocol|
|FC protocol for TOMM40 antibody 18409-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Nuo (Verified Customer) (01-28-2023)
Good antibody for WB with high titer.
Süleyman (Verified Customer) (01-23-2022)
TOMM40 antibody (Green) is used 1:100 dilution (2 hour at RT) and 1:700 of Alexa Fluor 488 secondary antibody (1 hour RT). 4% Paraformaldehyde fixation for 10 min at RT. Permeabilization with 0.3% Triton X for 10-15 minutes at RT. 5% FBS in PBS used for blocking for 1 hour at RT. The image is colocalized with TOMM20 (Red). Blue is DAPI. As you can see there is colocalization with TOMM20 with TOMM40. It is a perfect colocalization. You might also reduce concentration to 1:300 as it was used low power of laser.
Tom (Verified Customer) (03-23-2021)
Cos7 cells fixed in 4% PFA and permeabilized in 0.1% Triton X-100. Cells blocked in 1% BSA in PBS.
Daniela (Verified Customer) (01-08-2020)
Immunoblot of total cell lysate of HeLa cell gives three bands, one specific for human Tom40 above 37 KDa on 15% acrylamide gel, the other two no specific and with much lower intensity. The anti-Tom40 antibody worked very well and specifically when conjugated with magnetic beads for immunoprecipitation.