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TOMM40 Polyclonal antibody
TOMM40 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 18409-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, human brain tissue, A431 cells, HeLa cells, HepG2 cells |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver tissue, human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 18 publications below |
IF | See 3 publications below |
Product Information
18409-1-AP targets TOMM40 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TOMM40 fusion protein Ag13065 |
Full Name | translocase of outer mitochondrial membrane 40 homolog (yeast) |
Calculated molecular weight | 38 kDa |
Observed molecular weight | 38 kDa |
GenBank accession number | BC017224 |
Gene symbol | TOMM40 |
Gene ID (NCBI) | 10452 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for TOMM40 antibody 18409-1-AP | Download protocol |
IHC protocol for TOMM40 antibody 18409-1-AP | Download protocol |
IF protocol for TOMM40 antibody 18409-1-AP | Download protocol |
IP protocol for TOMM40 antibody 18409-1-AP | Download protocol |
FC protocol for TOMM40 antibody 18409-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell Reciprocal Roles of Tom7 and OMA1 during Mitochondrial Import and Activation of PINK1. | ||
Neurobiol Dis Amyloid beta-mediated KIF5A deficiency disrupts anterograde axonal mitochondrial movement. | ||
Nat Commun The class 3 PI3K coordinates autophagy and mitochondrial lipid catabolism by controlling nuclear receptor PPARα. | ||
Cancers (Basel) TOM40 Inhibits Ovarian Cancer Cell Growth by Modulating Mitochondrial Function Including Intracellular ATP and ROS Levels.
| ||
Toxicon Changes in the mitochondrial proteome in human hepatocytes in response to alpha-amanitin hepatotoxicity. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Daniela (Verified Customer) (01-08-2020) | Immunoblot of total cell lysate of HeLa cell gives three bands, one specific for human Tom40 above 37 KDa on 15% acrylamide gel, the other two no specific and with much lower intensity. The anti-Tom40 antibody worked very well and specifically when conjugated with magnetic beads for immunoprecipitation.
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