Torsin A Polyclonal antibody

Torsin A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10296-1-AP

Synonyms

DQ2, Dystonia 1 protein, DYT1, TOR1A, Torsin 1A, Torsin A, Torsin family 1 member A, TorsinA

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Validation Data Gallery

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  WB analysis of mouse brain using 10296-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10296-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10296-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10296-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10296-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10296-1-AP (Torsin A antibody) at dilution of 1:100 (under 25x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells, mouse kidney tissue, mouse liver tissue, rat brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10296-1-AP targets Torsin A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Torsin A fusion protein Ag0358
• Full Name: torsin family 1, member A (torsin A)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 42-50 kDa
• GenBank Accession Number: BC000674
• Gene Symbol: TOR1A
• Gene ID (NCBI): 1861
• RRID: AB_2206116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOR1A (torsin A), also known as DYT1, is a member of AAA+ family of ATPases that mediate chaperone and other functions involved in conformational modeling of proteins, protection from stress, and targeting of proteins to cellular organelles. TOR1A is a 37 kDa ATP binding protein highly expressed in hippocampus, spinal cord, kidney and liver. TOR1A primarily localizes to the ER while exposure to oxidative stress causes the redistribution to cell surface protrusions. Mutations in gene encoding TOR1A cause the most severe form of hereditary dystonia, termed early onset, generalized dystonia. Interestingly, Lewy bodies of PD patients were intensely immunoreactive for torsinA.

Publications

Species	Application	Title
mouse	WB	Neurobiol Dis; Postnatal Foxp2 regulates early psychiatric-like phenotypes and associated molecular alterations in the R6/1 transgenic mouse model of Huntington's disease; Authors - Ened Rodríguez-Urgellés