TPI1 Polyclonal antibody

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  WB analysis using 10713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10713-1-AP

  WB result of TPI1 antibody (10713-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPI1 transfected HEK-293 cells.

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  WB analysis of HepG2 using 10713-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10713-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10713-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10713-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 10713-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 10713-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 10713-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10713-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10713-1-AP

  IP result of anti-TPI1 (IP:10713-1-AP, 3ug; Detection:10713-1-AP 1:1000) with HEK-293 cells lysate 2500ug.

• Featured Product
• KD/KO Validated

TPI1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 10713-1-AP

Publications(42)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

Triose-phosphate isomerase, TPI, TIM, Methylglyoxal synthase, EC:5.3.1.1

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, HepG2 cells, rat liver tissue, rat kidney tissue, RAW 264.7 cells, mouse liver tissue, mouse kidney tissue, NIH/3T3 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 38 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

10713-1-AP targets TPI1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, yeast, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1175

  Product name: Recombinant human TPI1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-249 aa of BC007086

  Sequence: MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ

• Full Name: triosephosphate isomerase 1
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC007086
• Gene Symbol: TPI1
• Gene ID (NCBI): 7167
• RRID: AB_2207716
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P60174
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell; The histone deacetylase Sirt6 regulates glucose homeostasis via Hif1alpha.; Authors - Zhong Lei L
		Nat Chem Biol; SF2312 is a natural phosphonate inhibitor of enolase.; Authors - Paul G Leonard
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma
human,mouse	WB,IHC,IF	Brain; Amyloid-dependent triosephosphate isomerase nitrotyrosination induces glycation and tau fibrillation.; Authors - Guix Francesc X FX