TPPII Polyclonal antibody

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  WB analysis of Jurkat using 14120-1-AP

  WB result of TPPII antibody (14120-1-AP, 1:1000) with si-control and si-TPPII transfected Jurkat cells.

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  WB analysis of Jurkat using 14120-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14120-1-AP (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14120-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14120-1-AP (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 14120-1-AP

  IP result of anti-TPPII (IP:14120-1-AP, 4ug; Detection:66017-1-Ig, 1:500) with Jurkat cells lysate 2400ug.

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  IP experiment of Jurkat using 14120-1-AP

  IP result of anti-TPPII (IP:14120-1-AP, 4ug; Detection:14120-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of human gliomas using 14120-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14120-1-AP (TPPII antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14120-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14120-1-AP (TPPII antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14120-1-AP

  Immunofluorescent analysis of HepG2 cells, using TPP2 antibody 14120-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 14120-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug TPPII Polyclonal antibody (14120-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

TPPII Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14120-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

TPP2, tripeptidyl peptidase II, EC:3.4.14.10, FLJ40359, TPP 2

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Tested Applications

• Positive WB detected in: Jurkat cells, Jurkat
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Product Information

14120-1-AP targets TPPII in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5296

  Product name: Recombinant human TPP2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 900-1249 aa of BC039905

  Sequence: LERLKDLPFIVSHRLSNTLSLDIHENHSFALLGKKKSSNLTLPPKYNQPFFVTSLPDDKIPKGAGPGCYLAGSLTLSKTELGKKADVIPVHYYLIPPPTKTKNGSKDKEKDSEKEKDLKEEFTEALRDLKIQWMTKLDSSDIYNELKETYPNYLPLYVARLHQLDAEKERMKRLNEIVDAANAVISHIDQTALAVYIAMKTDPRPDAATIKNDMDKQKSTLVDALCRKGCALADHLLHTQAQDGAISTDAEGKEEEGESPLDSLAETFWETTKWTDLFDNKVLTFAYKHALVNKMYGRGLKFATKLVEEKPTKENWKNCIQLMKLLGWTHCASFTENWLPIMYPPDYCVF

• Full Name: tripeptidyl peptidase II
• Calculated Molecular Weight: 1249 aa, 138 kDa
• Observed Molecular Weight: 135-140 kDa
• GenBank Accession Number: BC039905
• Gene Symbol: TPPII
• Gene ID (NCBI): 7174
• RRID: AB_2206028
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P29144
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Cell Sci; Unveiling a novel serpinB2-tripeptidyl peptidase II signaling axis during senescence.; Authors - Chia-Li Liao ; KD Validated
human	WB	J Immunol; Cytosolic Processing Governs TAP-Independent Presentation of a Critical Melanoma Antigen.; Authors - Nathalie Vigneron
human	IF	Mol Cell Biochem; Novel protein-protein interactions of TPPII, p53, and SIRT7.; Authors - Jarmila Nahálková
human	WB,IF	Genes Cancer; Tripeptidyl Peptidase II Is Required for c-MYC-Induced Centriole Overduplication and a Novel Therapeutic Target in c-MYC-Associated Neoplasms.; Authors - Stefan Duensing ; KD Validated
human	WB	J Immunol; The Activation State of CD4 T Cells Alters Cellular Peptidase Activities, HIV Antigen Processing, and MHC Class I Presentation in a Sequence-Dependent Manner.; Authors - Julie Boucau