TRAPPC2 Polyclonal antibody, PBS Only

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  WB analysis using 12484-1-AP (same clone as 12484-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12484-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12484-1-AP (same clone as 12484-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12484-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 12484-1-AP (same clone as 12484-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12484-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 12484-1-AP (same clone as 12484-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12484-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human lung using 12484-1-AP (same clone as 12484-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12484-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 12484-1-AP (same clone as 12484-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12484-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 12484-1-AP (same clone as 12484-1-PBS)

  IP result of anti-TRAPPC2 (IP:12484-1-AP, 3ug; Detection:12484-1-AP 1:500) with PC-3 cells lysate 2800ug. This data was developed using the same antibody clone with 12484-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 12484-1-AP (same clone as 12484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12484-1-AP (TRAPPC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12484-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 12484-1-AP (same clone as 12484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12484-1-AP (TRAPPC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12484-1-PBS in a different storage buffer formulation.

TRAPPC2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12484-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

MBP-1-interacting protein 2A, MIP-2A, SEDLP1, Trafficking protein particle complex subunit 2B, TRAPPC2.19

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Product Information

12484-1-PBS targets TRAPPC2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3201

  Product name: Recombinant human TRAPPC2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-140 aa of BC016915

  Sequence: MSGSFYFVIVGHHDNPVFEMEFLPAGKAESKDDHRHLNQFIAHAALDLVDENMWLSNNMYLKTVDKFNEWFVSAFVTAGHMRFIMLHDIRQEDGIKNFFTDVYDLYIKFSMNPFYEPNSPIRSSAFDRKVQFLGKKHLLS

• Full Name: trafficking protein particle complex 2
• Calculated Molecular Weight: 140 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC016915
• Gene Symbol: TRAPPC2
• Gene ID (NCBI): 6399
• RRID: AB_2208136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P0DI82
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRAPPC2 is one component of the TRAPP multisubunit tethering complex involved in intracellular vesicle trafficking [PMID:21525244]. It acts as an adaptor for the TRAPP complex in mammalian cells, mediating interactions with both TRAPPC9 and TRAPPC8. It prevents transcriptional repression and induction of cell death by ENO1. Also it plays a role in vesicular transport from endoplasmic reticulum to Golgi.