Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
28392-1-AP targets TRIM3 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28823 Product name: Recombinant human TRIM3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 187-324 aa of NM_001248006 Sequence: SQQLQERKAEALAQISAAFEDLEQALQQRKQALVSDLETICGAKQKVLQSQLDTLRQGQEHIGSSCSFAEQALRLGSAPEVLLVRKHMRERLAALAAQAFPERPHENAQLELVLEVDGLRRSVLNLGALLTTSATAHE Predict reactive species |
| Full Name | tripartite motif-containing 3 |
| Calculated Molecular Weight | 81 kDa |
| Observed Molecular Weight | 75 kDa, 80-85 kDa |
| GenBank Accession Number | NM_001248006 |
| Gene Symbol | TRIM3 |
| Gene ID (NCBI) | 10612 |
| RRID | AB_2881131 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75382 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM3, a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM family of genes is largely studied because of their roles in development, differentiation and host cell antiviral defenses. TRIM3 is a mammalian tumor suppressor whose loss can increase the incidence and promote the development of GBM(PMID: 23318451). TRIM3 has four isoforms with the MW of 67-80 kDa and can be detected as 75, 80, 82,85 kDa after posttranslational modification(PMID: 24947043/PMID: 24086586 ).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM3 antibody 28392-1-AP | Download protocol |
| IHC protocol for TRIM3 antibody 28392-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Microbiol TRIM3 attenuates cytokine storm caused by Dabie bandavirus via promoting Toll-like receptor 3 degradation | ||
Mol Cell Biochem Tripartite-motif 3 represses ovarian cancer progression by downregulating lactate dehydrogenase A and inhibiting AKT signaling |
