Tested Applications
| Positive WB detected in | A549 cells, mouse colon tissue, NIH/3T3 cells, HeLa cells |
| Positive IP detected in | U-251 cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse embryo tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:100-1:400 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10326-1-AP targets TRIM32 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0401 Product name: Recombinant human TRIM32 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 111-430 aa of BC003154 Sequence: FCRSCGLVLCEPCREADHQPPGHCTLPVKEAAEERRRDFGEKLTRLRELMGELQRRKAALEGVSKDLQARYKAVLQEYGHEERRVQDELARSRKFFTGSLAEVEKSNSQVVEEQSYLLNIAEVQAVSRCDYFLAKIKQADVALLEETADEEEPELTASLPRELTLQDVELLKVGHVGPLQIGQAVKKPRTVNVEDSWAMEATASAASTSVTFREMDMSPEEVVASPRASPAKQRGPEAASNIQQCLFLKKMGAKGSTPGMFNLPVSLYVTSQGEVLVADRGNYRIQVFTRKGFLKEIRRSPSGIDSFVLSFLGADLPNLT Predict reactive species |
| Full Name | tripartite motif-containing 32 |
| Calculated Molecular Weight | 72 kDa |
| Observed Molecular Weight | 70-72 kDa |
| GenBank Accession Number | BC003154 |
| Gene Symbol | TRIM32 |
| Gene ID (NCBI) | 22954 |
| RRID | AB_2208005 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13049 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390). Western blot analysis detected TRIM32 at an apparent molecular mass of 70-72 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for TRIM32 antibody 10326-1-AP | Download protocol |
| IF protocol for TRIM32 antibody 10326-1-AP | Download protocol |
| IHC protocol for TRIM32 antibody 10326-1-AP | Download protocol |
| IP protocol for TRIM32 antibody 10326-1-AP | Download protocol |
| WB protocol for TRIM32 antibody 10326-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-TRIM32 (Cat# 10326-1-AP) has 22 citations in journals including Nature Communications, Molecular Cell, Science Advances, Cell Death & Differentiation, PNAS, Translational Psychiatry, FASEB Journal, iScience, and PLoS Pathogens. Research fields encompass oncology (HCC, breast, gastric, glioma, NSCLC, HNSCC), neurology (fear memory, Purkinje cells, motor function), virology (coronavirus, VEEV), cell biology (autophagy, ubiquitination, STING signaling), immunology, and muscular dystrophy.
| Species | Application | Title |
|---|---|---|
Nat Commun Small intestinal γδ T17 cells promote SAE through STING/C1q-induced microglial synaptic pruning in male mice | ||
Mol Cell Opposing regulation of the STING pathway in hepatic stellate cells by NBR1 and p62 determines the progression of hepatocellular carcinoma | ||
Sci Adv An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis. | ||
Cell Death Differ TRIM32 promotes anoikis resistance and metastasis in NSCLC by degrading CHEK2 to enhance IL-6 secretion
| ||
Int J Biol Sci TRIM32 promotes neuronal ferroptosis by enhancing K63-linked ubiquitination and subsequent p62-selective autophagic degradation of GPX4
| ||
J Am Soc Nephrol Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis. |
Reviews
What customers say
The single review for this anti-Trim32 antibody (used in Western Blot at a 1:1000 dilution) is highly positive, rated 5 stars. The reviewer reported long-standing difficulty detecting Trim32 knockdown/knockout at the protein level despite trying several antibodies, even though transcript-level downregulation was clear. This antibody resolved the issue, allowing clear visualization of the expected protein reduction. It was tested on heart, liver, and bat samples. The reviewer strongly recommends the product.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ashraf (Verified Customer) (08-22-2024) | We have so far struggled with detecting the knockdown/knockout of Trim32 in our experimental settings despite testing several antibodies. We could very clearly see downregulation or absence of Trim32 as expected at transcript level but failed to detect the same at protein level. However, use of Trim32 Ab from Proteintech has unbelievably solved this issue! Highly highly recommended *****
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