Validation Data Gallery
|Positive WB detected in||HEK-293 cells|
|Positive IHC detected in||human lymphoma tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE1 cells, mouse embryo tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10326-1-AP targets TRIM32 in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TRIM32 fusion protein Ag0401|
|Full Name||tripartite motif-containing 32|
|Calculated molecular weight||72 kDa|
|Observed molecular weight||65-72 kDa|
|GenBank accession number||BC003154|
|Gene ID (NCBI)||22954|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390).
Generation of the short TRIM32 isoform is regulated by Lys 247 acetylation and a PEST sequence.
Biochem Biophys Res Commun
Knockdown of TRIM32 inhibits tumor growth and increases the therapeutic sensitivity to temozolomide in glioma in a p53-dependent and -independent manner.
Front Aging Neurosci
Deficiency of TRIM32 Impairs Motor Function and Purkinje Cells in Mid-Aged Mice.
J Cell Mol Med
TRIM32 promotes cell proliferation and invasion by activating β-catenin signalling in gastric cancer.
A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.
J Cell Sci
TRIM32, but not its muscular dystrophy-associated mutant, positively regulates and is targeted to autophagic degradation by p62/SQSTM1.