• Featured Product
  • KD/KO Validated

TRIM32 Polyclonal antibody

TRIM32 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10326-1-AP

Synonyms

72 kDa Tat interacting protein, BBS11, HT2A, LGMD2H, TATIP, TRIM32, tripartite motif containing 32, Zinc finger protein HT2A



Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman lymphoma tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, mouse embryo tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10326-1-AP targets TRIM32 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM32 fusion protein Ag0401
Full Name tripartite motif-containing 32
Calculated molecular weight 72 kDa
Observed molecular weight 65-72 kDa
GenBank accession numberBC003154
Gene symbol TRIM32
Gene ID (NCBI) 22954
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390).

Protocols

Product Specific Protocols
WB protocol for TRIM32 antibody 10326-1-APDownload protocol
IHC protocol for TRIM32 antibody 10326-1-APDownload protocol
IF protocol for TRIM32 antibody 10326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Biochem Biophys Res Commun

Knockdown of TRIM32 inhibits tumor growth and increases the therapeutic sensitivity to temozolomide in glioma in a p53-dependent and -independent manner.

Authors - Yu Cai
  • KD Validated
humanWB,IHC

J Cell Mol Med

TRIM32 promotes cell proliferation and invasion by activating β-catenin signalling in gastric cancer.

Authors - Changming Wang
  • KD Validated
mouseWB

Hum Mol Genet

Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component.

Authors - Kudryashova Elena E
  • KO Validated