• Featured Product
  • KD/KO Validated

TRIM32 Polyclonal antibody

TRIM32 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10326-1-AP

Synonyms

72 kDa Tat interacting protein, BBS11, HT2A, LGMD2H, TATIP, TRIM32, tripartite motif containing 32, Zinc finger protein HT2A



Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman lymphoma tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, mouse embryo tissue
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10326-1-AP targets TRIM32 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM32 fusion protein Ag0401
Full Name tripartite motif-containing 32
Calculated molecular weight 72 kDa
Observed molecular weight 65-72 kDa
GenBank accession numberBC003154
Gene symbol TRIM32
Gene ID (NCBI) 22954
RRIDAB_2208005
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390).

Protocols

Product Specific Protocols
WB protocol for TRIM32 antibody 10326-1-APDownload protocol
IHC protocol for TRIM32 antibody 10326-1-APDownload protocol
IF protocol for TRIM32 antibody 10326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.

Authors - Molly M Wilson
mouse,humanIHC

J Am Soc Nephrol

Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis.

Authors - Sybille Koehler
mouseWB

Hum Mol Genet

Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component.

Authors - Kudryashova Elena E
  • KO Validated
human,ratWB,IF,IP

FASEB J

Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL.

Authors - Lei Liu
  • KD Validated
humanIF

J Cell Sci

TRIM32, but not its muscular dystrophy-associated mutant, positively regulates and is targeted to autophagic degradation by p62/SQSTM1.

Authors - Katrine Stange Overå
humanWB

iScience

A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.

Authors - Sheng Li