|Positive WB detected in||COLO 320 cells, MCF-7 cells, PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Sample-dependent, check data in validation data gallery|
55374-1-AP targets TRIM33 in WB, IF applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Full Name||tripartite motif-containing 33|
|Calculated molecular weight||122 kDa|
|Observed molecular weight||140-150 kDa|
|GenBank accession number||NM_015906|
|Gene ID (NCBI)||51592|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
TRIM33, also named as KIAA1113, RFG7 and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight of detected is about 140-150 kDa as the same as the result from the paper(PMID:25639486;26592194).