TRIM37 antibody (13037-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 13037-1-AP (same clone as 13037-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.
WB analysis using 13037-1-AP (same clone as 13037-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.
WB analysis using 13037-1-AP (same clone as 13037-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 13037-1-AP (same clone as 13037-1-PBS)
IP result of anti-TRIM37 (IP:13037-1-AP, 4ug; Detection:13037-1-AP 1:800) with mouse brain tissue lysate 1800 ug. This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 13037-1-AP (same clone as 13037-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 13037-1-AP (same clone as 13037-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 13037-1-AP (same clone as 13037-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 13037-1-AP (same clone as 13037-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using TRIM37 antibody (13037-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.
IF Staining of U-87 MG using 13037-1-AP (same clone as 13037-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using TRIM37 antibody (13037-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

13037-1-PBS targets TRIM37 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRIM37 fusion protein Ag3690 Predict reactive species
Full Name	tripartite motif-containing 37
Calculated Molecular Weight	964 aa, 108 kDa
Observed Molecular Weight	130 kDa
GenBank Accession Number	BC036012
Gene Symbol	TRIM37
Gene ID (NCBI)	4591
RRID	AB_10640800
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O94972
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TRIM37 (tripartite motif containing 37) is a member of the tripartite motif (RING, B-Box, and coiled-coil domains) family . Its gene mutations result in mulibrey nanism, which is a rare, autosomal-recessive growth disorder of prenatal onset, with characteristic dysmorphic features, pericardial constriction, and hepatomegaly .TRIM37 is located on chromosome 17q23, encoding a protein with an estimated molecular weight of 130 kDa or 89kDa. (PMID: 28098873, PMID: 29940807, PMID: 14757854)