Tested Applications
| Positive WB detected in | mouse brain tissue, MCF-7 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse testis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13037-1-AP targets TRIM37 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3690 Product name: Recombinant human TRIM37 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 122-450 aa of BC036012 Sequence: GMHGGHTFKPLAEIYEQHVTKVNEEVAKLRRRLMELISLVQEVERNVEAVRNAKDERVREIRNAVEMMIARLDTQLKNKLITLMGQKTSLTQETELLESLLQEVEHQLRSCSKSELISKSSEILMMFQQVHRKPMASFVTTPVPPDFTSELVPSYDSATFVLENFSTLRQRADPVYSPPLQVSGLCWRLKVYPDGNGVVRGYYLSVFLELSAGLPETSKYEYRVEMVHQSCNDPTKNIIREFASDFEVGECWGYNRFFRLDLLANEGYLNPQNDTVILRFQVRSPTFFQKSRDQHWYITQLEAAQTSYIQQINNLKERLTIELSRTQKS Predict reactive species |
| Full Name | tripartite motif-containing 37 |
| Calculated Molecular Weight | 964 aa, 108 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC036012 |
| Gene Symbol | TRIM37 |
| Gene ID (NCBI) | 4591 |
| RRID | AB_10640800 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94972 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM37 (tripartite motif containing 37) is a member of the tripartite motif (RING, B-Box, and coiled-coil domains) family . Its gene mutations result in mulibrey nanism, which is a rare, autosomal-recessive growth disorder of prenatal onset, with characteristic dysmorphic features, pericardial constriction, and hepatomegaly .TRIM37 is located on chromosome 17q23, encoding a protein with an estimated molecular weight of 130 kDa or 89kDa. (PMID: 28098873, PMID: 29940807, PMID: 14757854)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM37 antibody 13037-1-AP | Download protocol |
| IHC protocol for TRIM37 antibody 13037-1-AP | Download protocol |
| IP protocol for TRIM37 antibody 13037-1-AP | Download protocol |
| WB protocol for TRIM37 antibody 13037-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRIM37 antibody (cat# 13037-1-AP) has accumulated 14 citations across journals including Cell Discovery, Science Advances, Cell Death Discovery, Molecular Medicine, Molecular Cancer Research, International Immunopharmacology, Translational Oncology, Cell Signaling, Brain Research Bulletin, Experimental Cell Research, PLoS ONE, and Biochemical and Biophysical Research Communications. Research fields span immunology, neurodegeneration (Huntington's disease, multiple sclerosis), oncology (endometrial, glioma, gallbladder, hepatocellular, lung cancers), infectious disease, liver injury, osteoarthritis, ferroptosis, and peripheral nerve injury.
| Species | Application | Title |
|---|---|---|
Cell Discov The TRIM37 variants in Mulibrey nanism patients paralyze follicular helper T cell differentiation | ||
Sci Adv TRIM37 is a primate-specific E3 ligase for Huntingtin and accounts for the striatal degeneration in Huntington's disease | ||
Cell Death Discov KIFC1 depends on TRIM37-mediated ubiquitination of PLK4 to promote centrosome amplification in endometrial cancer | ||
Microbiol Res Dynamic regulation of K33- and K48-linked ubiquitination of Tip60 by TRIM37 orchestrates host DNA damage response during Pseudomonas aeruginosa infection and recovery. | ||
Mol Med TRIM37 exacerbates hepatic ischemia/reperfusion injury by facilitating IKKγ translocation
| ||
Mol Cancer Res Downregulation of BASP1 promotes temozolomide resistance in gliomas via epigenetic activation of the FBXO32/NF-κB/MGMT axis |
