TRPV4 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84091-4-RR (same clone as 84091-4-PBS)

  Rat brain tissue(heated at 100 ° C and unboilded)lysates were subjected to SDS PAGE followed by western blot with 84091-4-RR (TRPV4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84091-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84091-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84091-4-RR against Human TRPV4 were performed. The affinity constant is 2.57 nM.

TRPV4 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 84091-4-PBS

Clone No.241143H1

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

Vanilloid receptor-like channel 2, TRP12, OTRPC4, Osm-9-like TRP channel 4, Osm 9 like TRP channel 4

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Product Information

84091-4-PBS targets TRPV4 in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag27253

  Product name: Recombinant human TRPV4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-210 aa of NM_001177428.1

  Sequence: MADSSEGPRAGPGEVAELPGDESGTPGGEAFPLSSLANLFEGEDGSLSPSPADASRPAGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHSSDNKRWRKKIIEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTI

• Full Name: transient receptor potential cation channel, subfamily V, member 4
• Calculated Molecular Weight: 98 kDa
• Observed Molecular Weight: 85~98 kDa
• GenBank Accession Number: NM_001177428.1
• Gene Symbol: TRPV4
• Gene ID (NCBI): 59341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9HBA0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRPV4, also called VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.