Multi-rAb® TRUB1 Multi-Recombinant antibody

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  WB analysis using RMX00073

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00073 (TRUB1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using RMX00073

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using RMX00073 (TRUB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using RMX00073

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using RMX00073 (TRUB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRUB1 Multi-Recombinant Antibody for WB, IHC, ELISA

Cat No. RMX00073

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

EC:5.4.99.-, EC:5.4.99.25, Pseudouridylate synthase TRUB1, Psi55 synthase TRUB1, PUS4

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Tested Applications

• Positive WB detected in: HEK-293T cells, HepG2 cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00073 targets TRUB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: TruB pseudouridine (psi) synthase homolog 1 (E. coli)
• Calculated Molecular Weight: 349 aa, 37 kDa
• Observed Molecular Weight: 37 kDa, 40 kDa
• GenBank Accession Number: BC030601
• Gene Symbol: TRUB1
• Gene ID (NCBI): 142940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q8WWH5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRUB1 gene encodes a 349-amino acid product, with a VFAVHKPKGPTSA box in positions 71-83 corresponding to motif I of the TruB family (probably involved in conserving protein structure). This protein may be responsible for synthesis of pseudouridine from uracil in transfer RNAs.