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TSG101 Polyclonal antibody
TSG101 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse and More (4)
Applications
WB, IP, IHC, CoIP, ELISA
Conjugate
Unconjugated
131
Cat no : 28283-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 131 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
28283-1-AP targets TSG101 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Reactivity | Human, Mouse |
Cited Reactivity | human, mouse, rat, goat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TSG101 fusion protein Ag28569 |
Full Name | tumor susceptibility gene 101 |
Calculated molecular weight | 44 kDa |
Observed molecular weight | 44 kDa |
GenBank accession number | BC002487 |
Gene symbol | TSG101 |
Gene ID (NCBI) | 7251 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for TSG101 antibody 28283-1-AP | Download protocol |
IHC protocol for TSG101 antibody 28283-1-AP | Download protocol |
IP protocol for TSG101 antibody 28283-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Extracell Vesicles A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells. | ||
Mol Ther Title: Extracellular vesicle-mediated delivery of anti-miR-106b inhibits morphine-induced primary ciliogenesis in the brain | ||
Redox Biol Small extracellular vesicles from senescent stem cells trigger adaptive mechanisms in young stem cells by increasing antioxidant enzyme expression | ||
Aging (Albany NY) Hair follicle mesenchymal stem cell exosomal lncRNA H19 inhibited NLRP3 pyroptosis to promote diabetic mouse skin wound healing | ||
J Control Release Sertoli cell-derived extracellular vesicles traverse the blood-testis barrier and deliver miR-24-3p inhibitor into germ cells improving sperm mobility |