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TSG101 Polyclonal antibody

TSG101 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (5)

Applications

WB,IP,IHC,ELISA

Conjugate

Unconjugated

Cat no : 28283-1-AP

Synonyms

Cell and organelle markers, ESCRT I complex subunit TSG101, Exosome marker, TSG10, TSG101, tumor susceptibility gene 101, VPS23



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28283-1-AP targets TSG101 in WB,IP,IHC,ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse, rat, goat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSG101 fusion protein Ag28569
Full Name tumor susceptibility gene 101
Calculated Molecular Weight 44 kDa
Observed Molecular Weight44 kDa
GenBank Accession NumberBC002487
Gene Symbol TSG101
Gene ID (NCBI) 7251
RRIDAB_2881104
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Protocols

Product Specific Protocols
WB protocol for TSG101 antibody 28283-1-APDownload protocol
IHC protocol for TSG101 antibody 28283-1-APDownload protocol
IP protocol for TSG101 antibody 28283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Authors - Zheng Fu
humanWB

J Extracell Vesicles

A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.

Authors - Charles P Hinzman
humanWB

Mol Ther

Title: Extracellular vesicle-mediated delivery of anti-miR-106b inhibits morphine-induced primary ciliogenesis in the brain

Authors - Rong Ma
humanWB

Redox Biol

Small extracellular vesicles from senescent stem cells trigger adaptive mechanisms in young stem cells by increasing antioxidant enzyme expression

Authors - Cristina Mas-Bargues
humanWB

Aging (Albany NY)

Hair follicle mesenchymal stem cell exosomal lncRNA H19 inhibited NLRP3 pyroptosis to promote diabetic mouse skin wound healing

Authors - Hongliang Yang
mouseWB

J Control Release

Sertoli cell-derived extracellular vesicles traverse the blood-testis barrier and deliver miR-24-3p inhibitor into germ cells improving sperm mobility

Authors - Yabing Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with 28283-1-AP (TSG101 antibody) at dilution of 1:10000 incubated at 4 degree C for 1.5 hours. TSG101 bands appeared at 42 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: Mouse liver tissue
FH

SONAM (Verified Customer) (01-09-2024)

Antibody works well. Ordered three times..

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: cancer cellls, cancer cell derived Exosome