TSG101 Polyclonal antibody

TSG101 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 28283-1-AP

Synonyms

ESCRT I complex subunit TSG101, TSG10, TSG101, tumor susceptibility gene 101, VPS23



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

28283-1-AP targets TSG101 in WB, IP, IHC,ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSG101 fusion protein Ag28569
Full Name tumor susceptibility gene 101
Calculated molecular weight 44 kDa
Observed molecular weight44 kDa
GenBank accession numberBC002487
Gene symbol TSG101
Gene ID (NCBI) 7251
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Protocols

Product Specific Protocols
WB protocol for TSG101 antibody 28283-1-APDownload protocol
IHC protocol for TSG101 antibody 28283-1-APDownload protocol
IP protocol for TSG101 antibody 28283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

KLF4-mediated upregulation of CD9 and CD81 suppresses hepatocellular carcinoma development via JNK signaling.

Authors - Yandong Li
mouseWB

Nat Metab

Islet transplantation in the subcutaneous space achieves long-term euglycaemia in preclinical models of type 1 diabetes.

Authors - Ming Yu
humanWB

J Proteome Res

Proteomic Analysis of Plasma sEVs Reveals That TNFAIP8 Is a New Biomarker of Cell Proliferation in Diabetic Retinopathy.

Authors - Jing Xiao
humanWB

Biochim Biophys Acta Mol Cell Res

IKKβ activation promotes amphisome formation and extracellular vesicle secretion in tumor cells.

Authors - Xueqiang Peng
humanWB

Stem Cell Res Ther

Therapeutic potential of small extracellular vesicles derived from lipoma tissue in adipose tissue regeneration-an in vitro and in vivo study.

Authors - Pengyu Hong
humanWB

Cell Res

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Authors - Zheng Fu