TSG101 Polyclonal antibody

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  WB analysis using 28283-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28283-1-AP (TSG101 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 28283-1-AP

  IP result of anti-TSG101 (IP:28283-1-AP, 4ug; Detection:28283-1-AP 1:2000) with HeLa cells lysate 1280 ug.

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  IHC staining of human lung cancer using 28283-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28283-1-AP (TSG101 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28283-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28283-1-AP (TSG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28283-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28283-1-AP (TSG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28283-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28283-1-AP (TSG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 28283-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TSG101 antibody (28283-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

TSG101 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 28283-1-AP

Publications(288)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

ESCRT-I complex subunit TSG101, TSG 10, TSG 101, TSG10, VPS 23

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 281 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

28283-1-AP targets TSG101 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, rabbit, monkey, chicken, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSG101 fusion protein Ag28569
• Full Name: tumor susceptibility gene 101
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC002487
• Gene Symbol: TSG101
• Gene ID (NCBI): 7251
• RRID: AB_2881104
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99816
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Publications

Species	Application	Title
human	WB	Cell Res; In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.; Authors - Zheng Fu
human	WB	J Extracell Vesicles; Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease; Authors - Rong Yang
human	WB	J Am Chem Soc; Efficient Metabolomics Profiling from Plasma Extracellular Vesicles Enables Accurate Diagnosis of Early Gastric Cancer; Authors - Fanqin Bu
human	WB	J Extracell Vesicles; A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.; Authors - Charles P Hinzman
human	WB	Mol Ther; Title: Extracellular vesicle-mediated delivery of anti-miR-106b inhibits morphine-induced primary ciliogenesis in the brain; Authors - Rong Ma
human	WB	Redox Biol; Small extracellular vesicles from senescent stem cells trigger adaptive mechanisms in young stem cells by increasing antioxidant enzyme expression; Authors - Cristina Mas-Bargues

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with 28283-1-AP (TSG101 antibody) at dilution of 1:10000 incubated at 4 degree C for 1.5 hours. TSG101 bands appeared at 42 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Mouse liver tissue

FH

SONAM (Verified Customer) (01-09-2024)

Antibody works well. Ordered three times..

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cellls, cancer cell derived Exosome