TTLL12 Monoclonal antibody

TTLL12 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H5C11

Cat no : 68044-1-Ig

Synonyms

dJ526I14.2, FLJ41795, KIAA0153, TTLL12

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Validation Data Gallery

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  WB analysis using 68044-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68044-1-Ig (TTLL12 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 68044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68044-1-Ig (TTLL12 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 68044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68044-1-Ig (TTLL12 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SKOV-3 cells, LNCap cells, PC-3 cells, A431 cells, HeLa cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human lung cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68044-1-Ig targets TTLL12 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TTLL12 fusion protein Ag13804
• Full Name: tubulin tyrosine ligase-like family, member 12
• Calculated Molecular Weight: 644 aa, 74 kDa
• Observed Molecular Weight: 72-74 kDa
• GenBank Accession Number: BC001070
• Gene Symbol: TTLL12
• Gene ID (NCBI): 23170
• RRID: AB_2918786
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.