TTRAP Polyclonal antibody

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  WB analysis of human spleen using 12203-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12203-1-AP (TTRAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12203-1-AP

  WB result of TTRAP antibody (12203-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTRAP transfected HepG2 cells.

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  WB analysis using 12203-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12203-1-AP (TTRAP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12203-1-AP

  IP result of anti-TTRAP (IP:12203-1-AP, 4ug; Detection:12203-1-AP 1:5000) with HepG2 cells lysate 1600 ug.

• Featured Product
• KD/KO Validated

TTRAP Polyclonal Antibody for WB, IP, ELISA

Cat No. 12203-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

EAP2, AD-022, AD022, 5'-tyrosyl-DNA phosphodiesterase, 5'-Tyr-DNA phosphodiesterase

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Tested Applications

• Positive WB detected in: HEK-293T cells, HepG2 cells, human spleen tissue, HeLa cells
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below

Product Information

12203-1-AP targets TTRAP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2846

  Product name: Recombinant human TTRAP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-362 aa of BC017553

  Sequence: MELGSCLEGGREAAEEEGEPEVKKRRLLCVEFASVASCDAAVAQCFLAENDWEMERALNSYFEPPVEESALERRPETISEPKTYVDLTNEETTDSTTSKISPSEDTQQENGSMFSLITWNIDGLDLNNLSERARGVCSYLALYSPDVIFLQEVIPPYYSYLKKRSSNYEIITGHEEGYFTAIMLKKSRVKLKSQEIIPFPSTKMMRNLLCVHVNVSGNELCLMTSHLESTRGHAAERMNQLKMVLKKMQEAPESATVIFAGDTNLRDREVTRCGGLPNNIVDVWEFLGKPKHCQYTWDTQMNSNLGITAACKLRFDRIFFRAAAEEGHIIPRSLDLLGLEKLDCGRFPSDHWGLLCNLDIIL

• Full Name: TRAF and TNF receptor associated protein
• Calculated Molecular Weight: 362 aa, 41 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC017553
• Gene Symbol: TTRAP
• Gene ID (NCBI): 51567
• RRID: AB_1607504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95551
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TTRAP is a 5-prime tyrosyl-DNA phosphodiesterase that is required for efficient repair of topoisomerase II-induced DNA double-strand breaks. Thus it can remove lots of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, generating a free 5' phosphate to DNA. It also has 3'-tyrosyl DNA phophodiesterase activity, but not so efficient as TDP1. TTRAP functions as a negative regulator of ETS1 and inhibit nuclear factor-kappa-B activation.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nucleic Acids Res; Reversal of DNA damage induced Topoisomerase 2 DNA-protein crosslinks by Tdp2.; Authors - Matthew J Schellenberg
mouse	WB	Cell Death Differ; NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.; Authors - Bin Zhang ; KO Validated
human	WB	Anal Biochem; Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase.; Authors - Graeme Thomson
human	WB	Int J Mol Sci; Combined PARP and Dual Topoisomerase Inhibition Potentiates Genome Instability and Cell Death in Ovarian Cancer; Authors - Inken Flörkemeier
mouse	WB	bioRxiv; DNA polymerase theta-mediated DNA repair is a functional dependency and therapeutic vulnerability in DNMT3A deficient leukemia cells; Authors - Bac Viet Le