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- KD/KO Validated
TTRAP Polyclonal antibody
TTRAP Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 12203-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human spleen tissue, HepG2 cells |
Positive IP detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 4 publications below |
Product Information
12203-1-AP targets TTRAP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TTRAP fusion protein Ag2846 |
Full Name | TRAF and TNF receptor associated protein |
Calculated Molecular Weight | 362 aa, 41 kDa |
Observed Molecular Weight | 49 kDa |
GenBank Accession Number | BC017553 |
Gene Symbol | TTRAP |
Gene ID (NCBI) | 51567 |
RRID | AB_1607504 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TTRAP is a 5-prime tyrosyl-DNA phosphodiesterase that is required for efficient repair of topoisomerase II-induced DNA double-strand breaks. Thus it can remove lots of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, generating a free 5' phosphate to DNA. It also has 3'-tyrosyl DNA phophodiesterase activity, but not so efficient as TDP1. TTRAP functions as a negative regulator of ETS1 and inhibit nuclear factor-kappa-B activation.
Protocols
Product Specific Protocols | |
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WB protocol for TTRAP antibody 12203-1-AP | Download protocol |
IP protocol for TTRAP antibody 12203-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nucleic Acids Res Reversal of DNA damage induced Topoisomerase 2 DNA-protein crosslinks by Tdp2. | ||
Cell Death Differ NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.
| ||
Anal Biochem Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase. | ||
Int J Mol Sci Combined PARP and Dual Topoisomerase Inhibition Potentiates Genome Instability and Cell Death in Ovarian Cancer |