Alpha Tubulin Polyclonal antibody

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  WB analysis using 16007-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16007-1-AP (Alpha Tubulin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat eye using 16007-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 16007-1-AP (Alpha Tubulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Alpha Tubulin Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16007-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, zebrafish, rat

Applications

WB, IHC, ELISA

Synonyms

alpha tubulin, Alpha tubulin 6, TUBA1C, TUBA6, Tubulin alpha 1C chain, Tubulin alpha 6 chain, tubulin, alpha 1c

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, zebrafish tissues, mouse ovary tissues
• Positive IHC detected in: rat eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16007-1-AP targets Alpha Tubulin in WB, IHC, ELISA applications and shows reactivity with human, mouse, zebrafish, rat samples.

• Tested Reactivity: human, mouse, zebrafish, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8695

  Product name: Recombinant human tubulin-Alpha protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-449 aa of BC004949

  Sequence: MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAVAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGADSADGEDEGEEY

• Full Name: tubulin, alpha 1c
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC004949
• Gene Symbol: Alpha Tubulin
• Gene ID (NCBI): 84790
• RRID: AB_2303957
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BQE3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.