Mouse TIMP2 Recombinant antibody, PBS Only (Capture/Detector)

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  WB analysis of A172 using 85882-2-RR (same clone as 85882-2-PBS)

  A172 cells were subjected to SDS PAGE followed by western blot with 85882-2-RR (Timp2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85882-2-PBS in a different storage buffer formulation.

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  WB analysis using 85882-2-RR (same clone as 85882-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85882-2-RR (Timp2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85882-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02176-3

  Sandwich ELISA standard curve of MP02176-3, Mouse Timp2 Recombinant Matched Antibody Pair - PBS only. 85882-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2960. 85882-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

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  Sample test of MP02176-3

  Mouse lung tissue (3 lungs in 1-2 mm pieces in 100 mL of medium) was cultured for 5 days in RPMI supplemented with 10% fetal bovine serum, 5 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse Timp2, and measured 53.04 ng/mL.

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  Sample test of MP02176-3

  Serum of sixteen mice was measured. The mouse Timp2 concentration of detected samples was determined to be 1,122.15 ng/mL with a range of 933.30 - 1,234.19 ng/mL.

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  Cytometric bead array standard curve of MP02176-2

  Cytometric bead array standard curve of MP02176-2, MOUSE Timp2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85882-3-PBS. Detection antibody: 85882-2-PBS. Standard: Eg2960. Range: 0.781-50 ng/mL.

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  Cytometric bead array standard curve of MP02176-1

  Cytometric bead array standard curve of MP02176-1, MOUSE Timp2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85882-2-PBS. Detection antibody: 85882-1-PBS. Standard: Eg2960. Range: 0.781-100 ng/mL.

TIMP2 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85882-2-PBS

Clone No.250231B8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Metalloproteinase inhibitor 2, Timp-2, Tissue inhibitor of metalloproteinases 2

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Product Information

85882-2-PBS targets TIMP2 as part of a matched antibody pair:

MP02176-1: 85882-2-PBS capture and 85882-1-PBS detection (validated in Cytometric bead array)

MP02176-2: 85882-3-PBS capture and 85882-2-PBS detection (validated in Cytometric bead array)

MP02176-3: 85882-4-PBS capture and 85882-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TIMP2 fusion protein Eg2960
• Full Name: tissue inhibitor of metalloproteinase 2
• Calculated Molecular Weight: 24 kDa
• GenBank Accession Number: uniprot无NM号
• Gene Symbol: Timp2
• Gene ID (NCBI): 21858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P25785
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The TIMPs are natural highly specific endogenous inhibitors of MMPs. Human TIMP family consists of four 21-28 kDa proteins known as TIMP1, TIMP2, TIMP3, and TIMP4 encoded by four paralogous genes.TIMPs are vital to the maintenance of ECM homeostasis primarily through their MMP inhibitory functions. TIMP2, a member of the TIMP family, regulates the proteolytic activity of all MMPs and is involved in cell differentiation, growth, migration, angiogenesis, and apoptosis. Urinary TIMP2 has been recently recognized as an early biomarker to predict Acute kidney injury (AKI) in critically ill patients.