TIMP2 Recombinant antibody

• 

  WB analysis of A172 using 85882-2-RR

  A172 cells were subjected to SDS PAGE followed by western blot with 85882-2-RR (Timp2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85882-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85882-2-RR (Timp2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

TIMP2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85882-2-RR

Clone No.250231B8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Metalloproteinase inhibitor 2, Timp-2, Tissue inhibitor of metalloproteinases 2

---

Tested Applications

• Positive WB detected in: A172 cells, mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85882-2-RR targets TIMP2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TIMP2 fusion protein Eg2960
• Full Name: tissue inhibitor of metalloproteinase 2
• Calculated Molecular Weight: 24 kDa
• GenBank Accession Number: uniprot无NM号
• Gene Symbol: Timp2
• Gene ID (NCBI): 21858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P25785
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TIMPs are natural highly specific endogenous inhibitors of MMPs. Human TIMP family consists of four 21-28 kDa proteins known as TIMP1, TIMP2, TIMP3, and TIMP4 encoded by four paralogous genes.TIMPs are vital to the maintenance of ECM homeostasis primarily through their MMP inhibitory functions. TIMP2, a member of the TIMP family, regulates the proteolytic activity of all MMPs and is involved in cell differentiation, growth, migration, angiogenesis, and apoptosis. Urinary TIMP2 has been recently recognized as an early biomarker to predict Acute kidney injury (AKI) in critically ill patients.