UBE2L3 Polyclonal antibody, PBS Only

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  WB analysis of HeLa using 14415-1-AP (same clone as 14415-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 14415-1-AP (same clone as 14415-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 14415-1-AP (same clone as 14415-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 14415-1-AP (same clone as 14415-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 14415-1-AP (same clone as 14415-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 14415-1-AP (same clone as 14415-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 14415-1-AP (same clone as 14415-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat kidney using 14415-1-AP (same clone as 14415-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 14415-1-AP (same clone as 14415-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 14415-1-AP (same clone as 14415-1-PBS)

  IP result of anti-UBE2L3 (IP:14415-1-AP, 3ug; Detection:14415-1-AP 1:2000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

UBE2L3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 14415-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

14415-1-PBS targets UBE2L3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5758

  Product name: Recombinant human UBE2L3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-154 aa of BC053368

  Sequence: MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD

• Full Name: ubiquitin-conjugating enzyme E2L 3
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC053368
• Gene Symbol: UBE2L3
• Gene ID (NCBI): 7332
• RRID: AB_2210891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P68036
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

UBE2L3(Ubiquitin-conjugating enzyme E2 L3) is also named as UBCE7, UBCH7 and belongs to the ubiquitin-conjugating enzyme family. It regulates nuclear hormone receptors transcriptional activity and may play a role in myelopoiesis.UBE2L3 is associated with susceptibility to systemic lupus erythematosus (SLE) and rheumatoid arthritis in European ancestry populations(PMID:22045845).