UBL4B Polyclonal antibody

UBL4B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14509-1-AP

Synonyms

Ubiquitin-like protein 4B, Ubiquitin like protein 4B, ubiquitin like 4B

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Validation Data Gallery

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  WB analysis of human testis using 14509-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14509-1-AP (UBL4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 14509-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14509-1-AP (UBL4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 14509-1-AP

  Immunofluorescent analysis of HepG2 cells, using UBL4B antibody 14509-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 14509-1-AP

  Immunofluorescent analysis of Hela cells, using UBL4B antibody 14509-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IF/ICC detected in: HepG2 cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14509-1-AP targets UBL4B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBL4B fusion protein Ag5968
• Full Name: ubiquitin-like 4B
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC058929
• Gene Symbol: UBL4B
• Gene ID (NCBI): 164153
• RRID: AB_10640905
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Andrology; The MAEL expression in mitochondria of human spermatozoa and the association with asthenozoospermia; Authors - Yu-Sheng Cheng