UBR4 Polyclonal antibody, PBS Only

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  WB analysis using 15966-1-AP (same clone as 15966-1-PBS)

  Various lysates were subjected to Tris-acetate gel system followed by western blot with 15966-1-AP (UBR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15966-1-AP (same clone as 15966-1-PBS)

  IP result of anti-UBR4 (IP:15966-1-AP, 4ug; Detection:15966-1-AP 1:2000) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 15966-1-AP (same clone as 15966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 15966-1-AP (same clone as 15966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 15966-1-AP (same clone as 15966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 15966-1-AP (same clone as 15966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 15966-1-AP (same clone as 15966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 15966-1-AP (same clone as 15966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15966-1-AP (same clone as 15966-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using UBR4 antibody (15966-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

UBR4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 15966-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

15966-1-PBS targets UBR4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8740

  Product name: Recombinant human UBR4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 451-799 aa of BC018694

  Sequence: GLTCCICREGYKFQPTKVLGIYTFTKRVALEELENKPRKQQGYSTVSHFNIVHYDCHLAAVRLARGREEWESAALQNANTKCNGLLPVWGPHVPESAFATCLARHNTYLQECTGQREPTYQLNIHDIKLLFLRFAMEQSFSADTGGGGRESNIHLIPYIIHTVLYVLNTTRATSREEKNLQGFLEQPKEKWVESAFEVDGPYYFTVLALHILPPEQWRATRVEILRRLLVTSQARAVAPGGATRLTDKAVKDYSAYRSSLLFWALVDLIYNMFKKVPTSNTEGGWSCSLAEYIRHNDMPIYEAADKALKTFQEEFMPVETFSEFLDVAGLLSEITDPESFLKDLLNSVP

• Full Name: ubiquitin protein ligase E3 component n-recognin 4
• Calculated Molecular Weight: 5183 aa, 570 kDa
• Observed Molecular Weight: 570 kDa, 270 kDa
• GenBank Accession Number: BC018694
• Gene Symbol: UBR4
• Gene ID (NCBI): 23352
• RRID: AB_2878202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5T4S7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

UBR4 (ubiquitin N-recognin domain-containing E3 ligase 4), also named P600, is an E3 ligase that can bind N-end rule substrates. UBR4-deficient mice die during embryogenesis and exhibit pleiotropic abnormalities (PMID: 23431188). UBR4 plays a role in membrane morphogenesis and extracellular matrix-mediated cell survival and signaling, human papillomavirus (HPV) E7-mediated cellular immortalization, and migration and development of neurons (PMID: 33658508, 25084275). The full-length UBR4 protein has a predicted size of ~570 kDa.