UCMA Polyclonal antibody

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  WB analysis of mouse spleen using 25503-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25503-1-AP (UCMA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

UCMA Polyclonal Antibody for WB, ELISA

Cat No. 25503-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

C10orf49, Gla rich protein, GRP, UCMA, Ucma C

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Tested Applications

• Positive WB detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25503-1-AP targets UCMA in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UCMA fusion protein Ag21985
• Full Name: upper zone of growth plate and cartilage matrix associated
• Calculated Molecular Weight: 138 aa, 17 kDa
• Observed Molecular Weight: ~14 kDa
• GenBank Accession Number: BC018068
• Gene Symbol: UCMA
• Gene ID (NCBI): 221044
• RRID: AB_2880106
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WVF2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCMA, also named as C10orf49, Ucma-C and GRP, is a 17 kDa small, highly charged, and secreted protein. It has been described as a novel secretory protein mainly expressed in cartilage and also as a novel vitamin-K-dependent protein. UCMA is involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells in peripheral zones of fetal cartilage and at the cartilage-bone interface.