Tested Applications
| Positive WB detected in | HepG2 cells, mouse pancreas tissue, rat pancreas tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16999-1-AP targets UFSP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10692 Product name: Recombinant human UFSP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 120-469 aa of BC010493 Sequence: MLEMSTSLAAVTPIIERESGGHHYVNMTLPVDAVISVAPEETWGKVRKLLVDAIHNQLTDMEKCILKYMKGTSIVVPEPLHFLLPGKKNLVTISYPSGIPDGQLQAYRKELHDLFNLPHDRPYFKRSNAYHFPDEPYKDGYIRNPHTYLNPPNMETGMIYVVQGIYGYHHYMQDRIDDNGWGCAYRSLQTICSWFKHQGYTERSIPTHREIQQALVDAGDKPATFVGSRQWIGSIEVQLVLNQLIGITSKILFVSQGSEIASQGRELANHFQSEGTPVMIGGGVLAHTILGVAWNEITGQIKFLILDPHYTGAEDLQVILEKGWCGWKGPDFWNKDAYYNLCLPQRPNMI Predict reactive species |
| Full Name | UFM1-specific peptidase 2 |
| Calculated Molecular Weight | 469 aa, 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC010493 |
| Gene Symbol | UFSP2 |
| Gene ID (NCBI) | 55325 |
| RRID | AB_2214070 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NUQ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ubiquitin-fold modifier 1 (Ufm1)-specific protease 2 (UfSP2) is a cysteine protease that is responsible for the release of Ufm1 from Ufm1-conjugated cellular proteins, as well as for the generation of mature Ufm1 from its precursor (PMID: 21228277). The MW of this protein is 53 kDa, and this antibody specially recognises the 53 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UFSP2 antibody 16999-1-AP | Download protocol |
| IP protocol for UFSP2 antibody 16999-1-AP | Download protocol |
| WB protocol for UFSP2 antibody 16999-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-UFL1 antibody (Cat# 16999-1-AP) has 14 citations. These appear in journals including Nature Communications, Nature Structural & Molecular Biology, Science Advances, Advanced Science, Cell Reports, International Journal of Biological Sciences, Genetics in Medicine, Cells, iScience, FASEB Journal, PLoS One, and Biochemical and Biophysical Research Communications. Research fields span ufmylation biology, DNA repair, AKT signaling, and cancer (breast, colorectal, prostate, lung), plus GPCR transport, ER homeostasis, neurodevelopmental disorders, and vascular smooth muscle biology.
| Species | Application | Title |
|---|---|---|
Nat Commun Systematic discovery of UFM1 receptors reveals a regulatory module in DNA repair directing non-homologous end-joining. | ||
Nat Commun The UFL1-AKT positive feedback loop promotes breast cancer progression by enhancing lipid synthesis. | ||
Nat Commun Targeting the UFL1-AKT cascade suppresses triple-negative breast cancer progression. | ||
Adv Sci (Weinh) UFL1-Mediated UFMylation of ENO1 Restrains Aerobic Glycolysis and Colorectal Cancer Progression | ||
Sci Adv The ufmylation cascade controls COPII recruitment, anterograde transport, and sorting of nascent GPCRs at ER
| ||
Int J Biol Sci Loss of UFL1 confers enzalutamide resistance of prostate tumors by regulating METTL16-mediated m6A modification of EEF1A1 mRNA. |
Reviews
What customers say
The single review for this product is highly positive, rated 5 stars. The reviewer used it for Western Blot on HEK293 cells at a 1:1000 dilution and reported that the antibody works extremely well and is very specific for detecting the UFSP2 protein. Overall, the feedback highlights strong performance and high specificity in its tested application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Isabelle Cristine (Verified Customer) (01-14-2022) | This antibody works extremely well and is very specific for detecting the UFSP2 protein in HEK293 cells
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