UFSP2 Polyclonal antibody

UFSP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 16999-1-AP

Synonyms

C4orf20, UFM1 peptidase 2, Ufm1 specific protease 2, UFSP2

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Validation Data Gallery

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  WB analysis of mouse pancreas using 16999-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16999-1-AP (UFSP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16999-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16999-1-AP (UFSP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16999-1-AP

  IP result of anti-UFSP2 (IP:16999-1-AP, 3ug; Detection:16999-1-AP 1:1000) with HepG2 cells lysate 1700ug.

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  IHC staining of human liver using 16999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16999-1-AP (UFSP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16999-1-AP (UFSP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 16999-1-AP

  Immunofluorescent analysis of HeLa cells using 16999-1-AP (UFSP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, NIH/3T3 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

16999-1-AP targets UFSP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UFSP2 fusion protein Ag10692
• Full Name: UFM1-specific peptidase 2
• Calculated Molecular Weight: 469 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC010493
• Gene Symbol: UFSP2
• Gene ID (NCBI): 55325
• RRID: AB_2214070
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin-fold modifier 1 (Ufm1)-specific protease 2 (UfSP2) is a cysteine protease that is responsible for the release of Ufm1 from Ufm1-conjugated cellular proteins, as well as for the generation of mature Ufm1 from its precursor (PMID: 21228277). The MW of this protein is 53 kDa, and this antibody specially recognises the 53 kDa protein.

Publications

Species	Application	Title
human,mouse	WB	Genet Med; A pathogenic UFSP2 variant in an autosomal recessive form of pediatric neurodevelopmental anomalies and epilepsy.; Authors - Min Ni
human	WB	Sci Adv; The ufmylation cascade controls COPII recruitment, anterograde transport, and sorting of nascent GPCRs at ER; Authors - Xin Xu ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Isabelle Cristine (Verified Customer) (01-14-2022)

This antibody works extremely well and is very specific for detecting the UFSP2 protein in HEK293 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

UFSP2 Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:16999-1-AP)