UGT1A1 Polyclonal antibody

UGT1A1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 23495-1-AP

Synonyms

GNT1, HUG BR1, UDPGT, UDPGT 1 1, UGT 1A, UGT1, UGT1 01, UGT1*1, UGT1.1, UGT1A, UGT1A1

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Validation Data Gallery

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  WB analysis using 23495-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23495-1-AP (UGT1A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

23495-1-AP targets UGT1A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UGT1A1 fusion protein Ag19001
• Full Name: UDP glucuronosyltransferase 1 family, polypeptide A1
• Calculated Molecular Weight: 533 aa, 60 kDa
• Observed Molecular Weight: 50-60 kDa, 65-70 kDa
• GenBank Accession Number: BC128415
• Gene Symbol: UGT1A1
• Gene ID (NCBI): 54658
• RRID: AB_2879286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UDP-glucuronosyltransferase 1A1 (UGT1A1) is one of nine transferases encoded on the UGT1 locus. Developmental regulation of the UGT1A1 gene is an important process that is needed to achieve normal levels of serum total bilirubin (TB). UGT1A1 is a 50-60 kda member of the UGT1A subfamily, and can be detected as 65-70kda band after posttranslational modification.

Publications

Species	Application	Title
human	WB,IF	J Mol Biol; Silencing of Aberrant Secretory Protein Expression by Disease-Associated Mutations.; Authors - Elena B Tikhonova
rat	WB,IHC	Phytomedicine; Oleanolic acid alleviates ANIT-induced cholestatic liver injury by activating Fxr and Nrf2 pathways to ameliorate disordered bile acids homeostasis.; Authors - Jianming Liu
human,rat	WB,IHC	Phytomedicine; NRF2 and FXR dual signaling pathways cooperatively regulate the effects of oleanolic acid on cholestatic liver injury; Authors - Jianming Liu
mouse	WB	Pathogens; Dysregulated Glucuronidation of Bilirubin Exacerbates Liver Inflammation and Fibrosis in Schistosomiasis Japonica through the NF-κB Signaling Pathway; Authors - Qingkai Xue
mouse	WB	Front Nutr; The Protective Effect of Sulforaphane on Dextran Sulfate Sodium-Induced Colitis Depends on Gut Microbial and Nrf2-Related Mechanism.; Authors - Canxia He
human	WB	Metabolites; The Glycosyltransferase Pathway: An Integrated Analysis of the Cell Metabolome; Authors - Yannick Audet-Delage