Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23495-1-AP targets UGT1A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19001 Product name: Recombinant human UGT1A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 26-263 aa of BC128415 Sequence: HAGKILLIPVDGSHWLSMLGAIQQLQQRGHEIVVLAPDASLYIRDGAFYTLKTYPVPFQREDVKESFVSLGHNVFENDSFLQRVIKTYKKIKKDSAMLLSGCSHLLHNKELMASLAESSFDVMLTDPFLPCSPIVAQYLSLPTVFFLHALPCSLEFEATQCPNPFSYVPRPLSSHSDHMTFLQRVKNMLIAFSQNFLCDVVYSPYATLASEFLQREVTVQDLLSSASVWLFRSDFVKD Predict reactive species |
| Full Name | UDP glucuronosyltransferase 1 family, polypeptide A1 |
| Calculated Molecular Weight | 533 aa, 60 kDa |
| Observed Molecular Weight | 50-60 kDa, 65-70 kDa |
| GenBank Accession Number | BC128415 |
| Gene Symbol | UGT1A1 |
| Gene ID (NCBI) | 54658 |
| RRID | AB_2879286 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P22309 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UDP-glucuronosyltransferase 1A1 (UGT1A1) is one of nine transferases encoded on the UGT1 locus. Developmental regulation of the UGT1A1 gene is an important process that is needed to achieve normal levels of serum total bilirubin (TB). UGT1A1 is a 50-60 kda member of the UGT1A subfamily, and can be detected as 65-70 kda band after posttranslational modification.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UGT1A1 antibody 23495-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
UGT1A1 Polyclonal antibody (Cat# 23495-1-AP) has 19 citations across journals including Int J Biol Sci, Phytomedicine, Acta Pharmacol Sin, FASEB J, Front Pharmacol, J Mol Biol, and others. Research fields span cholestatic liver injury, bile acid metabolism, drug-induced hepatotoxicity, gut microbiome interactions, cancer, inflammation, and bioartificial liver systems. Cited applications include WB, IHC, and IF in human, mouse, and rat models.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Schisandrin B Targets PXR to Enhance Bile Acid Metabolism and Alleviate ANIT-Induced Cholestatic Liver Injury via Dual Pathways. | ||
NPJ Biofilms Microbiomes Gut bacteria and the host synergies promote resveratrol metabolism and induce tolerance in ALD mice | ||
Phytomedicine Oleanolic acid alleviates ANIT-induced cholestatic liver injury by activating Fxr and Nrf2 pathways to ameliorate disordered bile acids homeostasis. | ||
Phytomedicine NRF2 and FXR dual signaling pathways cooperatively regulate the effects of oleanolic acid on cholestatic liver injury | ||
Acta Biomater Peptide-dendrimer-reinforced bioinks for 3D bioprinting of heterogeneous and biomimetic in vitro models | ||
Acta Pharmacol Sin PXR ribosylation at E194 amplifies NAPQI in acetaminophen‒induced liver injury in mice, rescued by Schisandrin B. |
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The user tested it on liver tissue at a 1:1000 dilution and reported that it "suits well for WB," indicating satisfactory performance for their intended use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (10-24-2025) | suits well for wb
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