Tested Applications
| Positive WB detected in | HEK-293 cells, mouse testis tissue, HeLa cells, HepG2 cells, Raji cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66898-1-Ig targets UPF1 in WB, IHC, IF/ICC, FC (Intra), RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28320 Product name: Recombinant human UPF1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 769-1118 aa of BC039817 Sequence: EAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSSMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY Predict reactive species |
| Full Name | UPF1 regulator of nonsense transcripts homolog (yeast) |
| Calculated Molecular Weight | 123 kDa |
| Observed Molecular Weight | 123-130 kDa |
| GenBank Accession Number | BC039817 |
| Gene Symbol | UPF1 |
| Gene ID (NCBI) | 5976 |
| RRID | AB_2882227 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q92900 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for UPF1 antibody 66898-1-Ig | Download protocol |
| IF protocol for UPF1 antibody 66898-1-Ig | Download protocol |
| IHC protocol for UPF1 antibody 66898-1-Ig | Download protocol |
| WB protocol for UPF1 antibody 66898-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
UPF1 Monoclonal antibody (3B6B5), Cat# 66898-1-Ig, has 15 total citations. Journals include Neuron, Nucleic Acids Research, Cell Reports, EMBO Reports, Molecular Medicine, Scientific Reports, Journal of Neuroscience, European Journal of Immunology, Journal of Molecular Biology, Cell Signaling, Journal of Cellular Physiology, International Journal of Medical Sciences, Functional & Integrative Genomics, and Gene. Research fields span nonsense-mediated mRNA decay, RNA splicing, neurodegeneration (ALS), cancer biology, sepsis, muscle regeneration, pain signaling, and cardiac genetics.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Widespread 3' UTR splicing regulates expression of oncogene transcripts through multiple mechanisms | ||
Mol Med CircMAPK1 induces cell pyroptosis in sepsis-induced lung injury by mediating KDM2B mRNA decay to epigenetically regulate WNK1 | ||
Cell Rep Inhibiting UPF1 methylation enhances tumor immunotherapy sensitivity by reducing nonsense-mediated mRNA decay | ||
Cell Rep A multimodal screening platform for endogenous dipeptide repeat proteins in C9orf72 patient iPSC neurons | ||
EMBO Rep O-GlcNAcylation promotes topoisomerase IIα catalytic activity in breast cancer chemoresistance |
Reviews
What customers say
The product has one verified review rated 5 out of 5 stars. The reviewer, Isabelle Barbosa, used it for Western Blot on HEK cells (lot 10021454) and described the performance as "Good." Overall, customer feedback is positive, though limited in volume.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
BARBOSA (Verified Customer) (01-30-2026) | Good
|
