Tested Applications
| Positive IHC detected in | human bladder tissue, mouse bladder tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
Product Information
21149-1-AP targets UPK2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15694 Product name: Recombinant human UPK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 41-168 aa of BC113900 Sequence: TESLLVALPPCHLTGGNATLMVRRANDSKVVTSSFVVPPCRGRRELVSVVDSGAGFTVTRLSAYQVTNLVPGTKFYISYLVKKGTATESSREIPMSTLPRRNMESIGLGMARTGGMVVITVLLSVAMF Predict reactive species |
| Full Name | uroplakin 2 |
| Calculated Molecular Weight | 184 aa, 19 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC113900 |
| Gene Symbol | UPK2 |
| Gene ID (NCBI) | 7379 |
| RRID | AB_10755290 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00526 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-2 (UPK2) is a single-pass type I membrane protein. UPK2 may play an important role in regulating the assembly of the AUM.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UPK2 antibody 21149-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biofabrication Construction of a three-dimensional urothelium on-chip with barrier function based on urinary flow microenvironment | ||
Sci Rep Direct conversion of fibroblasts into urothelial cells that may be recruited to regenerating mucosa of injured urinary bladder. | ||
Int J Mol Sci Involvement of Mast-Cell-Tryptase- and Protease-Activated Receptor 2-Mediated Signaling and Urothelial Barrier Dysfunction with Reduced Uroplakin II Expression in Bladder Hyperactivity Induced by Chronic Bladder Ischemia in the Rat | ||
Adv Sci (Weinh) In Situ Transformable Nanoparticle Effectively Suppresses Bladder Cancer by Damaging Mitochondria and Blocking Mitochondrial Autophagy Flux | ||
Exp Cell Res Mapping the initial effects of carcinogen-induced oncogenic transformation in the mouse bladder | ||
Biomark Res S100A9 as a potential novel target for experimental autoimmune cystitis and interstitial cystitis/bladder pain syndrome |
