- Featured Product
- KD/KO Validated
p115, USO1 Polyclonal antibody
p115, USO1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13509-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, human brain tissue, mouse testis tissue, mouse thymus tissue, SH-SY5Y cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 10 publications below |
| IF | See 9 publications below |
Product Information
13509-1-AP targets p115, USO1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | p115, USO1 fusion protein Ag4431 |
| Full Name | USO1 homolog, vesicle docking protein (yeast) |
| Calculated molecular weight | 961 aa, 115 kDa |
| Observed molecular weight | 115 kDa |
| GenBank accession number | BC032654 |
| Gene symbol | USO1 |
| Gene ID (NCBI) | 8615 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for p115, USO1 antibody 13509-1-AP | Download protocol |
| IHC protocol for p115, USO1 antibody 13509-1-AP | Download protocol |
| IF protocol for p115, USO1 antibody 13509-1-AP | Download protocol |
| IP protocol for p115, USO1 antibody 13509-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Sci GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation.
| ||
Bioorg Med Chem Inhibition of diverse opportunistic viruses by structurally optimized retrograde trafficking inhibitors. | ||
J Cell Sci The Arf GEF GBF1 and Arf4 synergize with the sensory receptor cargo, rhodopsin, to regulate ciliary membrane trafficking. | ||
Front Mol Neurosci Herpes Simplex Virus 1-Induced Blood-Brain Barrier Damage Involves Apoptosis Associated With GM130-Mediated Golgi Stress. | ||
Sci Rep Focused CRISPR-Cas9 genetic screening reveals USO1 as a vulnerability in B-cell acute lymphoblastic leukemia. |
