p115, USO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

p115, USO1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 13509-1-AP

Synonyms

P115, p115, USO1, Protein USO1 homolog, TAP, USO1, VDP, Vesicle docking protein

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Validation Data Gallery

• 

  WB analysis of HepG2 using 13509-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13509-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13509-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13509-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13509-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 13509-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13509-1-AP

  IP Result of anti-p115, USO1 (IP:13509-1-AP, 3ug; Detection:13509-1-AP 1:1500) with mouse brain tissue lysate 7000ug.

• 

  IHC staining of human gliomas using 13509-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13509-1-AP (p115, USO1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13509-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13509-1-AP (p115, USO1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13509-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (13509-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13509-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13509-1-AP (p115, USO1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13509-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13509-1-AP (p115, USO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13509-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (13509-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, human brain tissue, mouse testis tissue, mouse thymus tissue, SH-SY5Y cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IF: See 10 publications below

Product Information

13509-1-AP targets p115, USO1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: p115, USO1 fusion protein Ag4431
• Full Name: USO1 homolog, vesicle docking protein (yeast)
• Calculated molecular weight: 962 aa, 108 kDa
• Observed molecular weight: 115 kDa
• GenBank accession number: BC032654
• Gene symbol: USO1
• Gene ID (NCBI): 8615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).

Publications

Species	Application	Title
mouse	WB,IF	J Cell Sci; GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation.; Authors - Emiliana Giacomello ; KD Validated
human	IF	Bioorg Med Chem; Inhibition of diverse opportunistic viruses by structurally optimized retrograde trafficking inhibitors.; Authors - Dhimant Desai
mouse	IF	J Cell Sci; The Arf GEF GBF1 and Arf4 synergize with the sensory receptor cargo, rhodopsin, to regulate ciliary membrane trafficking.; Authors - Jing Wang
mouse	WB,IF	Front Mol Neurosci; Herpes Simplex Virus 1-Induced Blood-Brain Barrier Damage Involves Apoptosis Associated With GM130-Mediated Golgi Stress.; Authors - Qiang He
mouse	WB,IF	PLoS One; Golgi disruption and early embryonic lethality in mice lacking USO1.; Authors - Kim Susie S
human	WB	Sci Rep; Focused CRISPR-Cas9 genetic screening reveals USO1 as a vulnerability in B-cell acute lymphoblastic leukemia.; Authors - Amit Kumar Jaiswal

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