WB result of p115, USO1 antibody (13509-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p115, USO1 transfected HeLa cells.
WB result of p115, USO1 antibody (13509-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p115, USO1 transfected HeLa cells.
WB analysis using 13509-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 13509-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 13509-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13509-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 13509-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 13509-1-AP
IP result of anti-p115, USO1 (IP:13509-1-AP, 3ug; Detection:13509-1-AP 1:1500) with mouse brain tissue lysate 7000ug.
IP result of anti-p115, USO1 (IP:13509-1-AP, 3ug; Detection:13509-1-AP 1:1500) with mouse brain tissue lysate 7000ug.
IHC staining of human gliomas using 13509-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13509-1-AP (p115, USO1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13509-1-AP (p115, USO1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 13509-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13509-1-AP (p115, USO1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13509-1-AP (p115, USO1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 13509-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (13509-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (13509-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13509-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (13509-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (13509-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13509-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13509-1-AP (p115, USO1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13509-1-AP (p115, USO1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13509-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13509-1-AP (p115, USO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13509-1-AP (p115, USO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HEK-293 cells, mouse testis tissue, mouse thymus tissue, HeLa cells, human brain tissue, SH-SY5Y cells, HepG2 cells
Positive IP detected in
mouse brain tissue
Positive IHC detected in
human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:8000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).
WB result of p115, USO1 antibody (13509-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p115, USO1 transfected HeLa cells.
WB analysis using 13509-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 13509-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 13509-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13509-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 13509-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human gliomas using 13509-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13509-1-AP (p115, USO1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 13509-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13509-1-AP (p115, USO1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of mouse brain using 13509-1-AP
IP result of anti-p115, USO1 (IP:13509-1-AP, 3ug; Detection:13509-1-AP 1:1500) with mouse brain tissue lysate 7000ug.
IF/ICC Figures
IF Staining of HeLa using 13509-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (13509-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13509-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (13509-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13509-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13509-1-AP (p115, USO1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13509-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13509-1-AP (p115, USO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
p115, USO1 Polyclonal antibody
Catalog Number
13509-1-AP
Citations
25
Dilutions
WB : 1:1000-1:8000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:500-1:2000
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
