Validation Data Gallery
|Positive WB detected in||HepG2 cells, HEK-293 cells, human brain tissue, mouse testis tissue, mouse thymus tissue, SH-SY5Y cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
13509-1-AP targets p115, USO1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||p115, USO1 fusion protein Ag4431|
|Full Name||USO1 homolog, vesicle docking protein (yeast)|
|Calculated molecular weight||962 aa, 108 kDa|
|Observed molecular weight||115 kDa|
|GenBank accession number||BC032654|
|Gene ID (NCBI)||8615|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).
|Product Specific Protocols|
|WB protocol for p115, USO1 antibody 13509-1-AP||Download protocol|
|IHC protocol for p115, USO1 antibody 13509-1-AP||Download protocol|
|IF protocol for p115, USO1 antibody 13509-1-AP||Download protocol|
|IP protocol for p115, USO1 antibody 13509-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Cell Sci
GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation.
Bioorg Med Chem
Inhibition of diverse opportunistic viruses by structurally optimized retrograde trafficking inhibitors.
J Cell Sci
The Arf GEF GBF1 and Arf4 synergize with the sensory receptor cargo, rhodopsin, to regulate ciliary membrane trafficking.
Front Mol Neurosci
Herpes Simplex Virus 1-Induced Blood-Brain Barrier Damage Involves Apoptosis Associated With GM130-Mediated Golgi Stress.
Golgi disruption and early embryonic lethality in mice lacking USO1.
Focused CRISPR-Cas9 genetic screening reveals USO1 as a vulnerability in B-cell acute lymphoblastic leukemia.