USP1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86453-1-RR (same clone as 86453-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86453-1-RR (USP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86453-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86453-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86453-1-RR against Human USP1 were performed. The affinity constant is 14.5 pM.

USP1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86453-1-PBS

Clone No.251219E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Deubiquitinating enzyme 1, EC:3.4.19.12, hUBP, Ubiquitin carboxyl-terminal hydrolase 1, Ubiquitin carboxyl-terminal hydrolase 1, N-terminal fragment

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Product Information

86453-1-PBS targets USP1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5686

  Product name: Recombinant human USP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 437-785 aa of BC050525

  Sequence: RTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDKGNFVVDQMCEIGKPEPLNEEEARGVVENYNDEEVSIRVGGNTQPSKVLNKKNVEAIGLLGGQKSKADYELYNKASNPDKVASTAFAENRNSETSDTTGTHESDRNKESSDQTGINISGFENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL

• Full Name: ubiquitin specific peptidase 1
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 110 kDa, 100 kDa
• GenBank Accession Number: BC050525
• Gene Symbol: USP1
• Gene ID (NCBI): 7398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O94782
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. It is also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases (PMID: 9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment (PMID: 18082604).