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  • KD/KO Validated

USP20 Polyclonal antibody

USP20 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17491-1-AP

Synonyms

Deubiquitinating enzyme 20, hVDU2, KIAA1003, LSFR3A, Ubiquitin thiolesterase 20, USP20, VDU2



Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human testis tissue, human brain tissue, human spleen tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17491-1-AP targets USP20 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP20 fusion protein Ag11590
Full Name ubiquitin specific peptidase 20
Calculated Molecular Weight 914 aa, 102 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC039593
Gene Symbol USP20
Gene ID (NCBI) 10868
RRIDAB_2212570
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP20(ubiquitin carboxyl-terminal hydrolase 20) is also named as KIAA1003, LSFR3A, VDU2 and belongs to the peptidase C19 family and the USP20/USP33 subfamily. It can specifically deubiquitinate and stabilize HIF1A and, therefore, increase expression of HIF-1alpha targeted genes, such as vascular endothelial growth factor (VEGF). It also plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2.

Protocols

Product Specific Protocols
WB protocol for USP20 antibody 17491-1-APDownload protocol
IHC protocol for USP20 antibody 17491-1-APDownload protocol
IP protocol for USP20 antibody 17491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Genes Dev

Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2.

Authors - Wenyang Li
  • KD Validated
humanIF, WB

J Cell Biol

Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.

Authors - Jacob A Culver
humanWB,IHC

Cell Death Differ

USP20 positively regulates tumorigenesis and chemoresistance through β-catenin stabilization.

Authors - Chenming Wu
  • KD Validated
humanWB

Diabetes

Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination.

Authors - Yuguo Niu
humanWB

Front Oncol

USP20 is a predictor of poor prognosis in colorectal cancer and associated with lymph node metastasis, immune infiltration and chemotherapy resistance

Authors - RuiRi Jin
  • KD Validated
humanWB,IP

Mol Ther Oncolytics

USP18 and USP20 restrict oHSV-1 replication in resistant human oral squamous carcinoma cell line SCC9 and affect the viability of SCC9 cells.

Authors - Ruitao Lu
  • KD Validated