USP20 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP20 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 17491-1-AP

Synonyms

Deubiquitinating enzyme 20, hVDU2, KIAA1003, LSFR3A, Ubiquitin thiolesterase 20, USP20, VDU2

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Validation Data Gallery

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  WB analysis of HeLa using 17491-1-AP

  WB result of USP20 antibody (17491-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP20 transfected HeLa cells.

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  WB analysis using 17491-1-AP

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 17491-1-AP (USP20 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17491-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17491-1-AP (USP20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17491-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17491-1-AP (USP20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17491-1-AP

  IP result of anti-USP20 (IP:17491-1-AP, 4ug; Detection:17491-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human kidney using 17491-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17491-1-AP (USP20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 17491-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17491-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 17491-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 17491-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 17491-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 17491-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 17491-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 17491-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 17491-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 17491-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human testis tissue, human brain tissue, human spleen tissue, human lung tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 15 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• IP: See 4 publications below

Product Information

17491-1-AP targets USP20 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP20 fusion protein Ag11590
• Full Name: ubiquitin specific peptidase 20
• Calculated Molecular Weight: 914 aa, 102 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC039593
• Gene Symbol: USP20
• Gene ID (NCBI): 10868
• RRID: AB_2212570
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP20(ubiquitin carboxyl-terminal hydrolase 20) is also named as KIAA1003, LSFR3A, VDU2 and belongs to the peptidase C19 family and the USP20/USP33 subfamily. It can specifically deubiquitinate and stabilize HIF1A and, therefore, increase expression of HIF-1alpha targeted genes, such as vascular endothelial growth factor (VEGF). It also plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2.

Publications

Species	Application	Title
human	WB,IHC	Genes Dev; Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2.; Authors - Wenyang Li ; KD Validated
human	IF, WB	J Cell Biol; Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.; Authors - Jacob A Culver
human	WB,IHC	Cell Death Differ; USP20 positively regulates tumorigenesis and chemoresistance through β-catenin stabilization.; Authors - Chenming Wu ; KD Validated
human	WB	Diabetes; Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination.; Authors - Yuguo Niu
human	WB	Front Oncol; USP20 is a predictor of poor prognosis in colorectal cancer and associated with lymph node metastasis, immune infiltration and chemotherapy resistance; Authors - RuiRi Jin ; KD Validated
human	WB,IP	Mol Ther Oncolytics; USP18 and USP20 restrict oHSV-1 replication in resistant human oral squamous carcinoma cell line SCC9 and affect the viability of SCC9 cells.; Authors - Ruitao Lu ; KD Validated