Validation Data Gallery
|Positive WB detected in||HeLa cells, RAW 264.7 cells, recombinant ptotein, HepG2 cells, HuH-7 cells|
|Positive IHC detected in||human breast cancer tissue, human pancreas tissue, mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||RAW 264.7 cells|
|Western Blot (WB)||WB : 1:300-1:600|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 17856-1-AP is USP21 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||USP21 fusion protein Ag12316|
|Full Name||ubiquitin specific peptidase 21|
|Calculated molecular weight||565 aa, 63 kDa|
|Observed molecular weight||63 kDa|
|GenBank accession number||BC090946|
|Gene ID (NCBI)||27005|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
USP21(Ubiquitin carboxyl-terminal hydrolase 21) is also named as USP23.The USPs belong to a subclass of the protein-deubiquitinating enzyme (DUB) superfamily that are categorized into five subclasses based on their ubiquitin-protease domains in the human genome and have been shown to be involved in a broad range of biological activities(PMID:19910467). USP21 is expressed at different levels in the tissues examined. The highest level of USP21 expression is found in the heart, pancreas, and skeletal muscle. USP21 messages could also be detected in the brain, placenta, liver, and kidney but are very low in the Lung through the northern blot(PMID:10799498).
Int J Mol Sci
PR-619, a General Inhibitor of Deubiquitylating Enzymes, Diminishes Cisplatin Resistance in Urothelial Carcinoma Cells through the Suppression of c-Myc: An In Vitro and In Vivo Study.
Cell Death Dis
RBM47/SNHG5/FOXO3 axis activates autophagy and inhibits cell proliferation in papillary thyroid carcinoma.
Deubiquitinating Enzyme USP21 Inhibits HIV-1 Replication by Downregulating Tat Expression.
Cell Death Dis
The deubiquitinase USP21 stabilizes MEK2 to promote tumor growth.
Oligomerization-driven MLKL ubiquitylation antagonizes necroptosis.