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  • KD/KO Validated

USP21 Polyclonal antibody

USP21 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17856-1-AP

Synonyms

Deubiquitinating enzyme 21, Ubiquitin thiolesterase 21, USP16, USP21, USP23



Tested Applications

Positive WB detected inHeLa cells, RAW 264.7 cells, recombinant ptotein, HepG2 cells, HuH-7 cells
Positive IHC detected inhuman breast cancer tissue, human pancreas tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17856-1-AP is USP21 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP21 fusion protein Ag12316
Full Name ubiquitin specific peptidase 21
Calculated molecular weight 565 aa, 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC090946
Gene symbol USP21
Gene ID (NCBI) 27005
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP21(Ubiquitin carboxyl-terminal hydrolase 21) is also named as USP23.The USPs belong to a subclass of the protein-deubiquitinating enzyme (DUB) superfamily that are categorized into five subclasses based on their ubiquitin-protease domains in the human genome and have been shown to be involved in a broad range of biological activities(PMID:19910467). USP21 is expressed at different levels in the tissues examined. The highest level of USP21 expression is found in the heart, pancreas, and skeletal muscle. USP21 messages could also be detected in the brain, placenta, liver, and kidney but are very low in the Lung through the northern blot(PMID:10799498).

Protocols

Product Specific Protocols
WB protocol for USP21 antibody 17856-1-APDownload protocol
IHC protocol for USP21 antibody 17856-1-APDownload protocol
IF protocol for USP21 antibody 17856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

Deubiquitinating Enzyme USP21 Inhibits HIV-1 Replication by Downregulating Tat Expression.

Authors - Wenying Gao
  • KD Validated
humanWB

Cell Death Dis

The deubiquitinase USP21 stabilizes MEK2 to promote tumor growth.

Authors - Wenjuan Li
  • KD Validated