USP5 Polyclonal antibody

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  WB analysis of mouse lung using 15158-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15158-1-AP

  WB result of USP5 antibody (15158-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells.

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  WB analysis of MDA-MB-453s using 15158-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15158-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 15158-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15158-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15158-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15158-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15158-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15158-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15158-1-AP

  IP result of anti-USP5 (IP:15158-1-AP, 4ug; Detection:15158-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• Featured Product
• KD/KO Validated

USP5 Polyclonal Antibody for WB, IP, ELISA

Cat No. 15158-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, ELISA

Synonyms

Deubiquitinating enzyme 5, EC:3.4.19.12, Isopeptidase T, ISOT, ISOT 1

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Tested Applications

• Positive WB detected in: mouse lung tissue, A549 cells, HeLa cells, human brain tissue, rat lung tissue, rat brain tissue, MDA-MB-453s cells
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

15158-1-AP targets USP5 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7252

  Product name: Recombinant human USP5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC005139

  Sequence: MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDF

• Full Name: ubiquitin specific peptidase 5 (isopeptidase T)
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 95-105 kDa
• GenBank Accession Number: BC005139
• Gene Symbol: USP5
• Gene ID (NCBI): 8078
• RRID: AB_2213856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P45974
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IP,IF	Neuron; The Deubiquitinating Enzyme USP5 Modulates Neuropathic and Inflammatory Pain by Enhancing Cav3.2 Channel Activity.; Authors - Agustin García-Caballero
human	WB	J Exp Med; A pathological missense mutation in the deubiquitinase USP5 leads to insensitivity to pain; Authors - Flavia T T Antunes
	WB	FASEB J; PPP2R1B is modulated by ubiquitination and is essential for spermatogenesis.; Authors - Mufeng Du
mouse	WB	Toxicology; Critical role of Cav3.2 T-type calcium channels in the peripheral neuropathy induced by bortezomib, a proteasome-inhibiting chemotherapeutic agent, in mice.; Authors - Shiori Tomita