USP54 Polyclonal antibody

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  WB analysis using 26707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26707-1-AP (USP54 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 26707-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26707-1-AP (USP54 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

USP54 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26707-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

C10orf29, Inactive ubiquitin carboxyl-terminal hydrolase 54, Inactive ubiquitin-specific peptidase 54

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Tested Applications

• Positive WB detected in: LNCaP cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26707-1-AP targets USP54 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25080

  Product name: Recombinant human USP54 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 377-725 aa of BC130633

  Sequence: DSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQHSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

• Full Name: ubiquitin specific peptidase 54
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC130633
• Gene Symbol: USP54
• Gene ID (NCBI): 159195
• RRID: AB_3669564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q70EL1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP54 is a ubiquitin-specific protease in the deubiquitinating enzyme (DUB) family that promotes the malignant progression of many cancers. As a deubiquitinating enzyme, USP54 maintains the stability of PLK4 protein through deubiquitination, which promotes centrosome amplification and GC progression. USP54 is also highly expressed in colorectal cancers, and is associated with proliferation, invasion and migration of colorectal cancer. In addition, USP54 plays a key role in the transformation of hormone-sensitive prostate cancer (HSPC) into chemoresistant prostate cancer (CRPC), and may serve as a potential therapeutic target in CRPC.