|Positive WB detected in||HepG2 cells, mouse skeletal muscle tissue, human kidney tissue, human brain tissue, HEK-293 cells, mouse liver tissue, mouse kidney tissue, rat kidney tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human colon cancer tissue, human liver cancer tissue, mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HEK-293 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15275-1-AP targets VAPA in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VAPA fusion protein Ag7393|
|Full Name||VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa|
|Calculated molecular weight||28 kDa|
|Observed molecular weight||33 kDa|
|GenBank accession number||BC002992|
|Gene ID (NCBI)||9218|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a 33-kDa, ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.
|Product Specific Protocols|
|WB protocol for VAPA antibody 15275-1-AP||Download protocol|
|IHC protocol for VAPA antibody 15275-1-AP||Download protocol|
|IF protocol for VAPA antibody 15275-1-AP||Download protocol|
|IP protocol for VAPA antibody 15275-1-AP||Download protocol|
|Click here to view our Standard Protocols|
The ER-embedded UBE2J1/RNF26 ubiquitylation complex exerts spatiotemporal control over the endolysosomal pathway.
Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.
J Cell Sci
A trap mutant reveals the physiological client spectrum of TRC40.
J Biol Chem
Phosphoproteomics Identified an NS5A Phosphorylation Site Involved in Hepatitis C Virus Replication.
The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.
CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wei (Verified Customer) (09-22-2020)
The antibody have high specific band in western blot, but not so good for heart sections.