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  • KD/KO Validated

VAPA Polyclonal antibody

VAPA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15275-1-AP

Synonyms

33 kDa VAMP associated protein, hVAP 33, VAMP A, VAMP associated protein A, VAP 33, VAP A, VAP33, VAPA



Tested Applications

Positive WB detected inHepG2 cells, mouse skeletal muscle tissue, human kidney tissue, human brain tissue, HEK-293 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, mouse heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15275-1-AP targets VAPA in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAPA fusion protein Ag7393
Full Name VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Calculated molecular weight 28 kDa
Observed molecular weight28-30 kDa
GenBank accession numberBC002992
Gene symbol VAPA
Gene ID (NCBI) 9218
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.

Protocols

Product Specific Protocols
WB protocol for VAPA antibody 15275-1-APDownload protocol
IHC protocol for VAPA antibody 15275-1-APDownload protocol
IF protocol for VAPA antibody 15275-1-APDownload protocol
IP protocol for VAPA antibody 15275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCOIP

Mol Cell

The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation.

Authors - Yan G Zhao
monkeyWB

Mol Cell

MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.

Authors - Christophe A C Freyre
mouseWB

J Clin Invest

Upregulation of Rubicon promotes autosis during myocardial ischemia/reperfusion injury.

Authors - Jihoon Nah

EMBO J

A selective ER-phagy exerts procollagen quality control via a Calnexin-FAM134B complex.

Authors - Alison Forrester
humanWB

EMBO J

CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.

Authors - Thaddaeus Mutugi Nthiga
monkeyWB, CoIP,IF

Curr Biol

The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.

Authors - Yan G Zhao
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (09-22-2020)

The antibody have high specific band in western blot, but not so good for heart sections.

  • Applications: Western Blot, Immunofluorescence
  • Cell Tissue Type: Mouse Heart