VARS2 antibody (15776-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 15776-1-AP (same clone as 15776-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.
WB analysis using 15776-1-AP (same clone as 15776-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15776-1-AP (VARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 15776-1-AP (same clone as 15776-1-PBS)
IP result of anti-VARS2 (IP:15776-1-AP, 4ug; Detection:15776-1-AP 1:500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 15776-1-AP (same clone as 15776-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 15776-1-AP (same clone as 15776-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 15776-1-AP (same clone as 15776-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 15776-1-AP (same clone as 15776-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15776-1-AP (VARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 15776-1-AP (same clone as 15776-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VARS2 antibody (15776-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15776-1-PBS in a different storage buffer formulation.

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Overview

Tested Applications
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Product Information

15776-1-PBS targets VARS2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VARS2 fusion protein Ag8460 Predict reactive species
Full Name	valyl-tRNA synthetase 2, mitochondrial (putative)
Calculated Molecular Weight	119 kDa, 57 kDa
Observed Molecular Weight	118 kDa
GenBank Accession Number	BC008844
Gene Symbol	VARS2
Gene ID (NCBI)	57176
RRID	AB_2272699
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5ST30
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

VARS2(Valine--tRNA ligase, mitochondrial) is also named as KIAA1885, VARS2L, VARSL and belongs to the class-I aminoacyl-tRNA synthetase family. The deduced 993-amino acid protein has an N-terminal mitochondrial targeting signal with a predicted cleavage site after residue 29. VARS2 has characteristics of a class I mitochondrial aminoacyl-tRNA synthetase, including a classical Rossmann fold.