CoraLite® Plus 488-conjugated VASH2 Monoclonal antibody

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  IF Staining of SKOV-3 using CL488-67753

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite® Plus 488 VASH2 antibody (CL488-67753, Clone: 3A11D10 ) at dilution of 1:200.

VASH2 Monoclonal Antibody for IF/ICC

Cat No. CL488-67753

Clone No.3A11D10

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

FLJ12505, RP11 275G3.1, VASH2, VASHL, vasohibin 2, Vasohibin like protein

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Tested Applications

• Positive IF/ICC detected in: SKOV-3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67753 targets VASH2 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VASH2 fusion protein Ag29985
• Full Name: vasohibin 2
• Calculated Molecular Weight: 355 aa, 40 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC028194
• Gene Symbol: VASH2
• Gene ID (NCBI): 79805
• RRID: AB_2919537
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q86V25
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VASH2, also named as vasohibin 2, is an important pro-angiogenesis factor in solid tumor. It has been reported that VASH2 is expressed in mononuclear cells mobilized from bone marrow to promote angiogenesis. VASH2 also plays a key role in axon formation. VASH2 is localized as a nuclear type(with 311 amino acid residues) and cytoplasmic type (with 355 amino acid residues and low abundance). Cytoplasmic VASH2 is associated with carcinoma angiogenesis, while nuclear VASH2 may be associated with cell proliferation. 67753-1-Ig antibody recognizes both the nuclear and cytoplasmic isoforms. (PMID:23615928; 19204325; 31235911; 26177649)