VCPIP1 Polyclonal antibody

VCPIP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17802-1-AP

Synonyms

DUBA3, KIAA1850, VCIP135, VCPIP1

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Validation Data Gallery

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  WB analysis of MCF-7 using 17802-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17802-1-AP (VCPIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 17802-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17802-1-AP (VCPIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17802-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17802-1-AP (VCPIP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 17802-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17802-1-AP (VCPIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17802-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17802-1-AP (VCPIP1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: MCF7 cells
• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• RIP: See 1 publications below

Product Information

The immunogen of 17802-1-AP is VCPIP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VCPIP1 fusion protein Ag12035
• Full Name: valosin containing protein (p97)/p47 complex interacting protein 1
• Calculated Molecular Weight: 1222 aa, 134 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC094799
• Gene Symbol: VCPIP1
• Gene ID (NCBI): 80124
• RRID: AB_10699873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Immunol Res; USP22 deubiquitinates CD274 to suppress anti-cancer immunity.; Authors - Xing Huang
human	RIP	Genes Dis; Downregulation of nc886 contributes to prostate cancer cell invasion and TGFβ1-induced EMT.; Authors - Ronghui Yang