CoraLite®594-conjugated VE-cadherin Monoclonal antibody

VE-cadherin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4F9B10

Cat no : CL594-66804

Synonyms

7B4, 7B4 antigen, Cadherin 5, CD144, CDH5, FLJ17376, Vascular endothelial cadherin, VE cadherin, VE-cadherin



Tested Applications

Positive IF detected inhuman breast cancer tissue, human tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66804 targets VE-cadherin in WB, IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VE-cadherin fusion protein Ag27501
Full Name cadherin 5, type 2 (vascular endothelium)
Calculated Molecular Weight 88 kDa
GenBank Accession NumberNM_001795
Gene Symbol CDH5
Gene ID (NCBI) 1003
RRIDAB_2920043
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). E-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).

Protocols

Product Specific Protocols
IF protocol for CL594 VE-cadherin antibody CL594-66804Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Biochem Biophys Res Commun

Irisin links Claudin-5 preservation and Mfn2-mediated mitochondrial dynamics to resist doxorubicin-induced cardiac endothelial damage

Authors - Jun He
humanIF

Biomacromolecules

Interpenetrating Polymer Network HA/Alg-RGD Hydrogel: An Equilibrium of Macroscopic Stability and Microscopic Adaptability for 3D Cell Growth and Vascularization

Authors - Yuanshan Liu
humanIF

Biochem Biophys Res Commun

Dulaglutide restores endothelial progenitor cell levels in diabetic mice and mitigates high glucose-induced endothelial injury through SIRT1-mediated mitochondrial fission

Authors - Xi Mei