Tested Applications
| Positive WB detected in | K-562 cells, mouse brain tissue |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
26781-1-AP targets VGF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24820 Product name: Recombinant human VGF protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 81-280 aa of BC063835 Sequence: ALAAVLLQALDRPASPPAPSGSQQGPEEEAAEALLTETVRSQTHSLPAPESPEPAAPPRPQTPENGPEASDPSEELEALASLLQELRDFSPSSAKRQQETAAAETETRTHTLTRVNLESPGPERVWRASWGEFQARVPERAPLPPPAPSQFQARMPDSGPLPETHKFGEGVSSPKTHLGEALAPLSKAYQGVAAPFPKAR Predict reactive species |
| Full Name | VGF nerve growth factor inducible |
| Calculated Molecular Weight | 615 aa, 67 kDa |
| Observed Molecular Weight | 67 kDa, 90-100 kDa |
| GenBank Accession Number | BC063835 |
| Gene Symbol | VGF |
| Gene ID (NCBI) | 7425 |
| RRID | AB_2880632 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15240 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VGF encodes a neuropeptide precursor with specific expression in a subpopulation of neuroendocrine cells, and it is upregulated by nerve growth factor. The expression of VGF is developmentally regulated in different areas of the brain (PubMed: 18406753). Exercise upregulated expression of VGF in mouse hippocampus, and a synthetic Vgf-derived peptide produced an antidepressant response in mice (PubMed: 18059283). VGF can be detected the precursor form of 90-100 kDa before proteolytic cleavage (PMID:24934694, PMID:10433233).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for VGF antibody 26781-1-AP | Download protocol |
| WB protocol for VGF antibody 26781-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Biol Cell Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites. | ||
Clin Epigenetics METTL3 exerts synergistic effects on m6A methylation and histone modification to regulate the function of VGF in lung adenocarcinoma | ||
J Exp Clin Cancer Res Hedgehog-Gli1-derived exosomal circ-0011536 mediates peripheral neural remodeling in pancreatic cancer by modulating the miR-451a/VGF axis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH James (Verified Customer) (09-15-2020) | Antibody works well, it is able to detect both GST-tagged VGF expressed in HeLa cell lysates, and HRV 3C protease cleaved VGF after IVT reaction and GST purification.
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