VGF Polyclonal antibody

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  WB analysis using 26781-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26781-1-AP (VGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of U2OS using 26781-1-AP

  U2OS lysates prepared and IP of VGF performed using 1.0 μg of 26781-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human brain using 26781-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26781-1-AP (VGF Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 26781-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26781-1-AP (VGF Antibody) at dilution of 1:200 (under 10x lens).

VGF Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 26781-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

SCG7, Neurosecretory protein VGF, Neuro-Endocrine Specific Protein VGF, Neuroendocrine regulatory peptide-2, Neuroendocrine regulatory peptide-1

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Tested Applications

• Positive WB detected in: K-562 cells, mouse brain tissue
• Positive IP detected in: U2OS cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

26781-1-AP targets VGF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VGF fusion protein Ag24820
• Full Name: VGF nerve growth factor inducible
• Calculated Molecular Weight: 615 aa, 67 kDa
• Observed Molecular Weight: 67 kDa, 90-100 kDa
• GenBank Accession Number: BC063835
• Gene Symbol: VGF
• Gene ID (NCBI): 7425
• RRID: AB_2880632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15240
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VGF encodes a neuropeptide precursor with specific expression in a subpopulation of neuroendocrine cells, and it is upregulated by nerve growth factor. The expression of VGF is developmentally regulated in different areas of the brain (PubMed: 18406753). Exercise upregulated expression of VGF in mouse hippocampus, and a synthetic Vgf-derived peptide produced an antidepressant response in mice (PubMed: 18059283). VGF can be detected the precursor form of 90-100 kDa before proteolytic cleavage (PMID:24934694, PMID:10433233).

Publications

Species	Application	Title
human	IF	Mol Biol Cell; Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites.; Authors - Erin M Masucci
human	WB	Clin Epigenetics; METTL3 exerts synergistic effects on m6A methylation and histone modification to regulate the function of VGF in lung adenocarcinoma; Authors - Kesong Shi
human	WB,IHC	J Exp Clin Cancer Res; Hedgehog-Gli1-derived exosomal circ-0011536 mediates peripheral neural remodeling in pancreatic cancer by modulating the miR-451a/VGF axis; Authors - Weiqi Dai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (09-15-2020)

Antibody works well, it is able to detect both GST-tagged VGF expressed in HeLa cell lysates, and HRV 3C protease cleaved VGF after IVT reaction and GST purification.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cell lysate IVT expression

VGF Antibody Western Blot validation (1:1000 dilution) in HeLa cell lysate IVT expression (Cat no:26781-1-AP)