VGF Polyclonal antibody

VGF Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 26781-1-AP


Neurosecretory protein VGF, VGF

Tested Applications

Positive WB detected inK-562 cells, mouse brain tissue
Positive IP detected inU2OS cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

26781-1-AP targets VGF in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VGF fusion protein Ag24820
Full Name VGF nerve growth factor inducible
Calculated molecular weight 615 aa, 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC063835
Gene symbol VGF
Gene ID (NCBI) 7425
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VGF encodes a neuropeptide precursor with specific expression in a subpopulation of neuroendocrine cells, and it is upregulated by nerve growth factor. The expression of VGF is developmentally regulated in different areas of the brain (PubMed: 18406753). Exercise upregulated expression of VGF in mouse hippocampus, and a synthetic Vgf-derived peptide produced an antidepressant response in mice (PubMed: 18059283). VGF can be detected the precursor form of 90 kDa before proteolytic cleavage (PMID:24934694, PMID:10433233).


Product Specific Protocols
WB protocol for VGF antibody 26781-1-APDownload protocol
IHC protocol for VGF antibody 26781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Biol Cell

Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites.

Authors - Erin M Masucci


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


James (Verified Customer) (09-15-2020)

Antibody works well, it is able to detect both GST-tagged VGF expressed in HeLa cell lysates, and HRV 3C protease cleaved VGF after IVT reaction and GST purification.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cell lysate IVT expression
VGF Antibody Western Blot validation (1:1000 dilution) in HeLa cell lysate IVT expression (Cat no:26781-1-AP)