VHL Polyclonal antibody

VHL Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 24756-1-AP


HRCA1, Protein G7, pVHL, RCA1, VHL, VHL1

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, Raji cells
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

24756-1-AP targets VHL in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VHL fusion protein Ag21447
Full Name von Hippel-Lindau tumor suppressor
Calculated molecular weight 172 aa, 20 kDa
Observed molecular weight 18-24 kDa
GenBank accession numberBC058831
Gene symbol VHL
Gene ID (NCBI) 7428
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VHL (von Hippel-Lindau tumor suppressor) gene was identified as the tumor suppressor gene whose germ line mutations are associated with the inherited von Hippel-Lindau cancer syndrome (PMID: 8603073, 10722748). VHL patients develop a wide variety of tumors including retinal angioma, central nervous system hemangioblastoma, pheochromocytoma, and renal clear cell carcinoma (PMID: 10722748). VHL localizes predominantly to the cytoplasmic compartment but engages in a dynamic nuclear-cytoplasmic shuttle (PMID: 8700833, 9891082, 12101228). VHL has 3 isoforms with the molecular mass of 24, 20 and 18 kDa.


Product Specific Protocols
WB protocol for VHL antibody 24756-1-APDownload protocol
IHC protocol for VHL antibody 24756-1-APDownload protocol
IF protocol for VHL antibody 24756-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Oncol

Hypoxia-Induced LncRNA-MIR210HG Promotes Cancer Progression By Inhibiting HIF-1α Degradation in Ovarian Cancer.

Authors - Ping Liu

Cell Death Discov

Septin4 promotes cardiomyocytes apoptosis by enhancing the VHL-mediated degradation of HIF-1α.

Authors - Shaojun Wu

Pathol Oncol Res

MDM2 promotes cancer cell survival through regulating the expression of HIF-1α and pVHL in retinoblastoma

Authors - Shouhua Zhang