VHL Polyclonal antibody

VHL Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 24756-1-AP

Synonyms

HRCA1, Protein G7, pVHL, RCA1, VHL, VHL1



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, Raji cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

CoIPSee 1 publications below

Product Information

The immunogen of 24756-1-AP is VHL Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VHL fusion protein Ag21447
Full Name von Hippel-Lindau tumor suppressor
Calculated molecular weight 172 aa, 20 kDa
Observed molecular weight 18-24 kDa
GenBank accession numberBC058831
Gene symbol VHL
Gene ID (NCBI) 7428
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VHL (von Hippel-Lindau tumor suppressor) gene was identified as the tumor suppressor gene whose germ line mutations are associated with the inherited von Hippel-Lindau cancer syndrome (PMID: 8603073, 10722748). VHL patients develop a wide variety of tumors including retinal angioma, central nervous system hemangioblastoma, pheochromocytoma, and renal clear cell carcinoma (PMID: 10722748). VHL localizes predominantly to the cytoplasmic compartment but engages in a dynamic nuclear-cytoplasmic shuttle (PMID: 8700833, 9891082, 12101228). VHL has 3 isoforms with the molecular mass of 24, 20 and 18 kDa.

Protocols

Product Specific Protocols
WB protocol for VHL antibody 24756-1-APDownload protocol
IF protocol for VHL antibody 24756-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
CoIP

Cell Death Discov

Septin4 promotes cardiomyocytes apoptosis by enhancing the VHL-mediated degradation of HIF-1α.

Authors - Shaojun Wu