Villin Monoclonal antibody

Villin Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

CloneNo.

2B7B9

Cat no : 66096-1-Ig

Synonyms

VIL1, villin 1, VIL, D2S1471, 2B7B9

---

Validation Data Gallery

• 

  WB analysis of human kidney using 66096-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 66096-1-Ig

  human colon tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human small intestine using 66096-1-Ig

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 66096-1-Ig

  IP result of anti-Villin (IP:66096-1-Ig, 4ug; Detection:66096-1-Ig 1:1000) with mouse kidney tissue lysate 6000ug.

• 

  IHC staining of human colon using 66096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66096-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66096-1-Ig (Villin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

---

Tested Applications

• Positive WB detected in: Human kidney, tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Product Information

66096-1-Ig targets Villin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Villin fusion protein Ag9637
• Full Name: villin 1
• Calculated Molecular Weight: 827aa,93 kDa; 826aa,93 kDa
• Observed Molecular Weight: 93-95 kDa
• GenBank Accession Number: BC017303
• Gene Symbol: Villin
• Gene ID (NCBI): 7429
• RRID: AB_2881495
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Villin 1 (VIL1) is a 95-kDa F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.

Publications

Species	Application	Title
mouse	IF	Gastroenterology; Stiffness Restricts the Stemness of the Intestinal Stem Cells and Skews Their Differentiation Towards Goblet Cells; Authors - Shijie He
mouse	IF	Theranostics; The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition.; Authors - Fangjun Yu
mouse	IHC	Oxid Med Cell Longev; Sitagliptin Alleviates Radiation-Induced Intestinal Injury by Activating NRF2-Antioxidant Axis, Mitigating NLRP3 Inf--lammasome Activation, and Reversing Gut Microbiota Disorder.; Authors - Shanshan Huang
human,mouse	IF	Inflamm Bowel Dis; Excessive Mitochondrial Fission Suppresses Mucosal Repair by Impairing Butyrate Metabolism in Colonocytes; Authors - Shi-Chen Fu
human	IF, IHC	J Pharmacol Sci; Syntaxin 3 interacts with serotonin transporter and regulates its function.; Authors - Serika Motoike
human	IHC	Int J Clin Exp Pathol; A four actin-binding protein signature model for poor prognosis of patients with esophageal squamous cell carcinoma.; Authors - Zhang-Mei Peng