Tested Applications
| Positive WB detected in | Human kidney, tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC-Autostainer detected in | human colorectal cancer tissue, human pancreas cancer tissue, human large intestine tissue, human appendicitis tissue, human kidney tissue, human stomach cancer tissue |
| Positive IHC detected in | human colorectal cancer tissue, human pancreas cancer tissue, human appendicitis tissue, human kidney tissue, human colon tissue, human large intestine tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC)-AUTOSTAINER | IHC-AUTOSTAINER : 1:2500-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66096-1-Ig targets Villin in WB, IHC, IHC-Autostainer, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, sheep |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9637 Product name: Recombinant human VIL1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC017303 Sequence: MTKLSAQVKGSLNITTPGLQIWRIEAMQMVPVPSSTFGSFFDGDCYIILAIHKTASSLSYDIHYWIGQDSSLDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESEAFRGYFKQGLVIRKGGVASGMKHVETNSYDVQRLLHVKGKRNVVAGEVEMSWKSFNRGDVFLLDLGKLIIQWNGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWT Predict reactive species |
| Full Name | villin 1 |
| Calculated Molecular Weight | 827aa,93 kDa; 826aa,93 kDa |
| Observed Molecular Weight | 93-95 kDa |
| GenBank Accession Number | BC017303 |
| Gene Symbol | Villin |
| Gene ID (NCBI) | 7429 |
| RRID | AB_2881495 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P09327 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Villin 1 (VIL1) is a 95-kDa F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Villin antibody 66096-1-Ig | Download protocol |
| IHC protocol for Villin antibody 66096-1-Ig | Download protocol |
| IP protocol for Villin antibody 66096-1-Ig | Download protocol |
| WB protocol for Villin antibody 66096-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Villin Monoclonal antibody (2B7B9), catalog number 66096-1-Ig, has 16 total citations. These appear in journals including Cell Metabolism, Gastroenterology, Theranostics, Free Radical Biology and Medicine, Oxidative Medicine and Cellular Longevity, Alimentary Pharmacology & Therapeutics, Journal of Ethnopharmacology, International Journal of Molecular Sciences, Inflammatory Bowel Diseases, Oncotarget, Journal of Integrative Medicine, Translational Oncology, Journal of Pharmacological Sciences, Journal of Toxicological Sciences, and International Journal of Clinical and Experimental Pathology. Research fields span liver disease, intestinal injury, gut barrier function, colorectal cancer, esophageal cancer, and mucosal repair.
| Species | Application | Title |
|---|---|---|
Cell Metab Hepatic FXR-FGF4 is required for bile acid homeostasis via an FGFR4-LRH-1 signal node under cholestatic stress | ||
Gastroenterology Stiffness Restricts the Stemness of the Intestinal Stem Cells and Skews Their Differentiation Towards Goblet Cells | ||
Theranostics The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition. | ||
Free Radic Biol Med 4-Octyl itaconate attenuates radiation-induced intestinal injury associated with ferroptosis inhibition and microbiota rebalance. | ||
Oxid Med Cell Longev Sitagliptin Alleviates Radiation-Induced Intestinal Injury by Activating NRF2-Antioxidant Axis, Mitigating NLRP3 Inf--lammasome Activation, and Reversing Gut Microbiota Disorder. | ||
Aliment Pharmacol Ther Serum Villin-1-A Novel Marker of Gut Barrier Damage in Acutely Decompensated Cirrhosis: A Cohort Study and Validation. |
