Vimentin Polyclonal antibody

• Featured Product
• KD/KO Validated

Vimentin Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 22031-1-AP

Synonyms

VIM

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Validation Data Gallery

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  WB analysis of HEK-293 using 22031-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22031-1-AP (VIM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22031-1-AP

  WB result of Vimentin antibody (22031-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Vimentin transfected A549 cells.

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  WB analysis of HeLa using 22031-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22031-1-AP (VIM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 22031-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22031-1-AP (VIM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 22031-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22031-1-AP (Vimentin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 22031-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22031-1-AP (Vimentin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 22031-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22031-1-AP (Vimentin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 22031-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22031-1-AP (Vimentin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human kidney using 22031-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (22031-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 22031-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (22031-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse heart tissue, A549 cells
• Positive IHC detected in: human ovary tumor tissue, human tonsillitis tissue, human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Product Information

22031-1-AP targets Vimentin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Vimentin fusion protein Ag16898
• Full Name: vimentin
• Calculated Molecular Weight: 466 aa, 54 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC000163
• Gene Symbol: VIM
• Gene ID (NCBI): 7431
• RRID: AB_11182825
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

Publications

Species	Application	Title
human	WB	Tissue Eng Part C Methods; Pretreatment with Inflammatory Factors Altered the Secretome of Human Amniotic Epithelial Cells; Authors - Wenjiao Cao
mouse	IF	Biochim Biophys Acta Mol Basis Dis; A novel Cdc42-YAP-fibronectin signaling axis regulates ameloblast differentiation during early enamel formation; Authors - Jiayi Zhang
human	WB	Dig Liver Dis; CTHRC1 serves as an indicator in biliary atresia for evaluating the stage of liver fibrosis and predicting prognosis; Authors - Zequan Ding
human	WB	Int J Clin Exp Pathol; miR-34b-5p suppresses the epithelial-mesenchymal transition and metastasis in endometrial cancer AN3CA cells by targeting ZEB1; Authors - Lulu Shi
mouse	IF	BMC Oral Health; Fibrin-konjac glucomannan-black phosphorus hydrogel scaffolds loaded with nasal ectodermal mesenchymal stem cells accelerated alveolar bone regeneration; Authors - Yin Zou
human	WB,IF	Am J Cancer Res; High glucose-induced NCAPD2 upregulation promotes malignant phenotypes and regulates EMT via the Wnt/β-catenin signaling pathway in HCC; Authors - Yuhua Mai