Vimentin Polyclonal antibody

Vimentin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22031-1-AP

Synonyms

vimentin



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse heart tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22031-1-AP is Vimentin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vimentin fusion protein Ag16898
Full Name vimentin
Calculated molecular weight 466 aa, 54 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC000163
Gene symbol VIM
Gene ID (NCBI) 7431
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Vimentin antibody 22031-1-APDownload protocol
IHC protocol for Vimentin antibody 22031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols